Pandey Renu, Saluja Daman
Medical Biotechnology Laboratory, Dr. B. R Ambedkar Center for Biomedical Research, University of Delhi, Delhi 110007, India.
Medical Biotechnology Laboratory, Dr. B. R Ambedkar Center for Biomedical Research, University of Delhi, Delhi 110007, India.
Anal Biochem. 2017 Oct 1;534:24-27. doi: 10.1016/j.ab.2017.07.007. Epub 2017 Jul 6.
Efficient electrophoretic separation of isolated total RNA utilizes chemicals and agents to aid in nuclease free environment. However cost, extensive pre-run processing protocols as well as toxic byproducts limit the usage of such protocols. Moreover, these treatments affect the overall electrophoretic results by altering the conductivity of the running buffer and weaken the gel strength. We here provide a protocol for RNA visualization that obviates these shortcomings by preparation of agarose gel with hydrogen peroxide using the regular TAE buffer. The simple, inexpensive protocol exhibits superior results in a horizontal agarose gel electrophoresis.
对分离出的总RNA进行高效电泳分离时，需利用化学物质和试剂营造无核酸酶的环境。然而，成本、繁琐的预运行处理方案以及有毒副产物限制了此类方案的使用。此外，这些处理会改变运行缓冲液的电导率，进而影响整体电泳结果，并削弱凝胶强度。在此，我们提供一种RNA可视化方案，该方案通过使用常规TAE缓冲液制备含过氧化氢的琼脂糖凝胶，避免了这些缺点。这个简单、低成本的方案在水平琼脂糖凝胶电泳中展现出卓越的效果。
