Luppi P H, Sakai K, Salvert D, Berod A, Jouvet M
J Comp Neurol. 1986 Feb 8;244(2):204-12. doi: 10.1002/cne.902440207.
The aim of the present study was to determine the exact origins of the dopaminergic hypothalamohypophyseal projections in the cat brain. For this purpose, we used a retrograde tracer technique with horseradish peroxidase (HRP) in conjunction with tyrosine hydroxylase (TH) immunohistochemistry as a marker for the dopaminergic neurons. After injections of the tracer into the neuro-intermediate lobe, a substantial number of HRP-labeled neurons was observed in the supraoptic and paraventricular neurosecretory nuclei. Furthermore, a cluster of HRP-positive neurons was found in the tuberal component of the periventricular nucleus where few, if any, neurosecretory magnocellular cells are identified. TH immunohistochemistry on the same sections further revealed that virtually all these HRP-containing neurons showed TH immunoreactivity. These double-labeled neurons were medium in size and fusiform or ovoid and appeared to belong to the A14 dopamine cell group. In addition to these medium-sized double-labeled neurons, a magnocellular type of double-labeled cell body was identified just adjacent to the organum vasculosum of the lamina terminalis and in and around the supraoptic and paraventricular nuclei. These double-labeled cells appeared to be members of the A14 and A15 dopamine cell groups. In conclusion, the present study indicated that the dopaminergic projections to the cat neurointermediate lobe might originate mainly in the medium-sized cells located in the tuberal periventricular nucleus and partly in the large-sized cells located in and around the supraoptic and paraventricular neurosecretory nuclei.
本研究的目的是确定猫脑中多巴胺能下丘脑 - 垂体投射的确切起源。为此，我们使用了辣根过氧化物酶（HRP）逆行示踪技术，并结合酪氨酸羟化酶（TH）免疫组织化学作为多巴胺能神经元的标记物。将示踪剂注射到神经中间叶后，在视上核和室旁神经分泌核中观察到大量HRP标记的神经元。此外，在室周核的结节部发现了一群HRP阳性神经元，在那里几乎没有（如果有的话）神经分泌大细胞被识别出来。对同一切片进行的TH免疫组织化学进一步显示，几乎所有这些含有HRP的神经元都表现出TH免疫反应性。这些双标记神经元大小中等，呈梭形或椭圆形，似乎属于A14多巴胺细胞群。除了这些中等大小的双标记神经元外，在终板血管器附近以及视上核和室旁核内及其周围还鉴定出一种大细胞类型的双标记细胞体。这些双标记细胞似乎是A14和A15多巴胺细胞群的成员。总之，本研究表明，猫神经中间叶的多巴胺能投射可能主要起源于位于结节室周核的中等大小细胞，部分起源于位于视上核和室旁神经分泌核内及其周围的大细胞。
