Alm-Kristiansen A H, Dalen G, Klinkenberg G, Bekk L, Thorkildsen L T, Waterhouse K E, Kommisrud E
Department of Natural Sciences and Technology, Inland Norway University of Applied Sciences, Hamar, Norway.
SpermVital AS, Hamar, Norway.
Reprod Domest Anim. 2017 Dec;52(6):1019-1024. doi: 10.1111/rda.13017. Epub 2017 Jul 9.
The SpermVital technology comprises embedding of spermatozoa within an alginate gel to facilitate release of sperm cells over a prolonged period in utero after AI. The aim of this study was to examine whether the survival time of spermatozoa is extended when applying this immobilization technology in combination with cryopreservation. Sperm cell survival (acrosome and plasma membrane integrity) was studied in vitro for 48 hr at physiological temperature. One dose of SpermVital (SV) semen was compared with single doses of Biladyl (B) processed semen as well as double doses of B (B double). B double was obtained by adding a second B dose the following day, thereby mimicking double AI. Furthermore, reproductive performance applying single early timed AI (TAI) with SV following oestrus synchronization was studied in a field trial. Double insemination (TAI on two consecutive days) with B semen served as control. Number of acrosome-intact live sperm cells decreased over time in vitro for all treatments (p < .05). There was no difference between SV sperm cell survival and B double after 24 hr (p > .05). However, after 48 hr, SV sperm cell survival was higher than B double (p < .05). Moreover, multivariate analysis showed that the outcome of single early TAI with SV was not significantly different from B double (p > .05). Likelihood of pregnancy and calving in the heifer group was higher than in the cow group (p < .05). These results imply that spermatozoa immobilized in alginate gel have prolonged survival.
精子活力技术包括将精子嵌入藻酸盐凝胶中，以便在人工授精后在子宫内长时间促进精子细胞的释放。本研究的目的是检验在将这种固定技术与冷冻保存相结合时，精子的存活时间是否会延长。在生理温度下，体外研究精子细胞存活情况（顶体和质膜完整性）达48小时。将一剂精子活力（SV）精液与单剂量的Biladyl（B）处理精液以及双剂量的B（B双剂）进行比较。B双剂是在第二天添加第二剂B获得的，从而模拟两次人工授精。此外，在一项田间试验中研究了发情同步后单次早期定时人工授精（TAI）使用SV的繁殖性能。使用B精液进行两次连续输精（连续两天进行TAI）作为对照。所有处理组中，体外顶体完整的活精子细胞数量随时间减少（p < 0.05）。24小时后，SV精子细胞存活率与B双剂之间无差异（p > 0.05）。然而，48小时后，SV精子细胞存活率高于B双剂（p < 0.05）。此外，多变量分析表明，单次早期TAI使用SV的结果与B双剂无显著差异（p > 0.05）。小母牛组的怀孕和产犊可能性高于母牛组（p < 0.05）。这些结果表明，固定在藻酸盐凝胶中的精子具有更长的存活时间。
