Yamamoto Y, Nagaoka K, Kamite Y, Watanabe G, Allen T, Stansfield F, Taya K
Department of Veterinary Medicine, Tokyo University of Agriculture and Technology, Tokyo, Japan.
The Elephant Research Unit, Save Valley Conservancy, Zimbabwe.
Reprod Domest Anim. 2017 Dec;52(6):1138-1141. doi: 10.1111/rda.13010. Epub 2017 Jul 9.
Elephant ovaries contain multiple corpora lutea (CLs) throughout pregnancy. Two CLs (P-1 and P-2) collected from a pregnant African elephant were used to investigate their origin and physiological state in this study. The mRNA expressions of prolactin receptor, CYP11A and inhibin betaB subunit were higher in P-2 than in P-1, while LHCGR and inhibin betaA subunit mRNA were higher in P-1 than in P-2. Protein expression of cleaved caspase-3 was detected in P-1 but not in P-2. These results suggest different origins for the two CLs in this one pregnant elephant, and we also demonstrated the production of bioactive prolactin by the elephant placenta.
在整个孕期，大象卵巢中含有多个黄体（CLs）。本研究使用从一头怀孕的非洲象收集的两个黄体（P-1和P-2）来研究它们的起源和生理状态。催乳素受体、CYP11A和抑制素βB亚基的mRNA表达在P-2中高于P-1，而促黄体生成素/绒毛膜促性腺激素受体（LHCGR）和抑制素βA亚基mRNA在P-1中高于P-2。在P-1中检测到了裂解的半胱天冬酶-3的蛋白表达，而在P-2中未检测到。这些结果表明这头怀孕大象体内的两个黄体起源不同，并且我们还证明了大象胎盘能产生生物活性催乳素。
