Supercharging SpyCatcher toward an intrinsically disordered protein with stimuli-responsive chemical reactivity.
作者信息
Cao Yang, Liu Dong, Zhang Wen-Bin
机构信息
Key Laboratory of Polymer Chemistry & Physics of Ministry of Education, Center for Soft Matter Science and Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China.
出版信息
Chem Commun (Camb). 2017 Aug 3;53(63):8830-8833. doi: 10.1039/c7cc04507g.
PMID:28692103
Abstract
We report a supercharged, intrinsically disordered protein, SpyCatcher(-), possessing stimuli-responsive reactivity toward SpyTag with tunable yields ranging from 4% to 98% depending on pH, temperature, ionic strength, etc. The CD and NMR studies reveal that the reaction occurs through a folded intermediate formed probably via a different mechanism from that of SpyCatcher.
摘要
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