Kang Jun-Gu, Lee Hee-Woo, Ko Sungjin, Chae Joon-Seok
Laboratory of Veterinary Internal Medicine, Research Institute and BK21 Program for Veterinary Science and College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
J Vet Sci. 2018 Jan 31;19(1):59-70. doi: 10.4142/jvs.2018.19.1.59.
Outer membrane proteins (OMPs) of Gram-negative bacteria constitute the first line of defense protecting cells against environmental stresses including chemical, biophysical, and biological attacks. Although the 43-kDa OMP (OMP43) is major porin protein among -derived OMPs, its function remains unreported. In this study, OMP43-deficient mutant (Δomp43) was generated to investigate OMP43 function. Interestingly, Δ43 exhibited weaker proliferative ability than that of wild-type (WT) . To study the differences in proteomic expression between WT and Δ43, two-dimensional gel electrophoresis-based proteomic analysis was performed. Based on Clusters of Orthologus Groups functional assignments, 12 proteins were associated with metabolism, 7 proteins associated with information storage and processing, and 3 proteins associated with cellular processing and signaling. By semi-quantitative reverse transcriptase polymerase chain reaction, increases in D, X, A, B, and ATPA mRNA expression and decreases in and A mRNA expression were confirmed in Δ43. In conclusion, this is the first report showing that a loss of OMP43 expression in leads to retarded proliferation. Furthermore, our proteomic data provide useful information for the further investigation of mechanisms related to the growth of
革兰氏阴性菌的外膜蛋白（OMPs）构成了保护细胞抵御包括化学、生物物理和生物攻击在内的环境压力的第一道防线。尽管43 kDa的OMP（OMP43）是衍生OMPs中的主要孔蛋白，但它的功能尚未见报道。在本研究中，构建了OMP43缺陷突变体（Δomp43）以研究OMP43的功能。有趣的是，Δ43的增殖能力比野生型（WT）弱。为了研究WT和Δ43之间蛋白质组表达的差异，进行了基于二维凝胶电泳的蛋白质组分析。根据直系同源簇功能分类，12种蛋白质与代谢相关，7种蛋白质与信息存储和处理相关，3种蛋白质与细胞加工和信号传导相关。通过半定量逆转录聚合酶链反应，证实了Δ43中D、X、A、B和ATPA mRNA表达增加，而和A mRNA表达减少。总之，这是首次报道表明中OMP43表达缺失导致增殖迟缓。此外，我们的蛋白质组数据为进一步研究与生长相关的机制提供了有用信息。
