Shun-Da Du, Shao-Hua Li, Bao Jin, Xin Lu, Xin-Ting Sang, Hua-Yu Yang, Shou-Xian Zhong, Yi-Lei Mao, Department of Liver Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
World J Gastroenterol. 2017 Jun 21;23(23):4278-4284. doi: 10.3748/wjg.v23.i23.4278.
To investigate the evaluation of neogalactosylalbumin (NGA) for liver function assessment based on positron emission tomography technology.
Female Kunming mice were assigned randomly to two groups: fibrosis group and normal control group. A murine hepatic fibrosis model was generated by intraperitoneal injection of 10% carbon tetrachloride (CCl) at 0.4 mL every 48 h for 42 d. F-labeled NGA ([F]FNGA) was synthesized and administered at a dosage of 3.7 MBq/mouse to both fibrosis mice and normal control mice. Distribution of [F]FNGA amongst organs was examined, and dynamic scanning was performed. Parameters were set up to compare the uptake of tracers by fibrotic liver and healthy liver. Serologic tests for liver function were also performed.
The liver function of the fibrosis model mice was significantly impaired by the use of CCl. In the fibrosis model mice, hepatic fibrosis was verified by naked eye assessment and pathological analysis. [F]FNGA was found to predominantly accumulate in liver and kidneys in both control group ( = 21) and fibrosis group ( = 23). The liver uptake ability (LUA), peak time (T), and uptake rate (LUR) of [F]FNGA between healthy liver ( = 8) and fibrosis liver ( = 10) were significantly different ( < 0.05, < 0.01, and < 0.05, respectively). LUA was significantly correlated with total serum protein level (TP) ( < 0.05). T was significantly correlated with both TP and glucose (Glu) concentration ( < 0.05 both), and LUR was significantly correlated with both total bile acid and Glu concentration ( < 0.01 and < 0.05, respectively).
[F]FNGA mainly accumulated in liver and remained for sufficient time. Functionally-impaired liver showed a significant different uptake pattern of [F]FNGA compared to the controls.
探讨正电子发射断层扫描技术评价新型半乳糖白蛋白（NGA）的肝功能。
将雌性昆明小鼠随机分为两组：纤维化组和正常对照组。通过腹腔注射 10%四氯化碳（CCl），每次 0.4 毫升，每 48 小时一次，共 42 天，建立小鼠肝纤维化模型。合成 F 标记的 NGA([F]FNGA)，并以 3.7MBq/只的剂量给予纤维化小鼠和正常对照组小鼠。检测器官中[F]FNGA 的分布，并进行动态扫描。设置参数比较纤维肝和正常肝对示踪剂的摄取。还进行了肝功能的血清学检测。
CCl 的使用显著损害了纤维化模型小鼠的肝功能。在纤维化模型小鼠中，通过肉眼评估和病理分析证实了肝纤维化。[F]FNGA 主要在对照组（ = 21）和纤维化组（ = 23）的肝脏和肾脏中积聚。健康肝脏（ = 8）和纤维化肝脏（ = 10）之间[F]FNGA 的肝摄取能力（LUA）、峰值时间（T）和摄取率（LUR）均有显著差异（<0.05、<0.01 和<0.05）。LUA 与总血清蛋白水平（TP）显著相关（<0.05）。T 与 TP 和葡萄糖（Glu）浓度显著相关（均<0.05），LUR 与总胆汁酸和 Glu 浓度显著相关（均<0.01 和<0.05）。
[F]FNGA 主要积聚在肝脏并停留足够长的时间。功能受损的肝脏对[F]FNGA 的摄取模式与对照组有显著差异。
