高选择性和高灵敏度的内源性过氧亚硝酸盐双光子荧光探针及其在活细胞和组织中的应用。
Highly Selective and Sensitive Two-Photon Fluorescence Probe for Endogenous Peroxynitrite Detection and Its Applications in Living Cells and Tissues.
机构信息
Department of Chemistry and Nano Science, Ewha Womans University , Seoul 120-750, Korea.
Department of Chemistry and Energy Systems Research, Ajou University , Suwon 443-749, Korea.
出版信息
Anal Chem. 2017 Aug 15;89(16):8496-8500. doi: 10.1021/acs.analchem.7b02059. Epub 2017 Jul 24.
A new two-photon fluorescence probe for endogenous peroxynitrite (ONOO) detection was designed and synthesized. The probe exhibits good selectivity and sensitivity for ONOO in phosphate-buffered saline solution with a low detection limit (3.5 × 10 M). Furthermore, the probe displays good performance in detecting endogenous ONOO, not only in RAW 264.7 cells but also in rat hippocampal tissue, with a high two-photon cross-section value (δ ≈ 100 GM) at a deep depth of 120 μm.
一种新的用于检测内源性过氧亚硝酸盐（ONOO ）的双光子荧光探针被设计和合成。该探针在磷酸盐缓冲溶液中对 ONOO 具有良好的选择性和灵敏度，检测限低（3.5×10 M）。此外，该探针在检测内源性 ONOO 方面表现出良好的性能，不仅在 RAW 264.7 细胞中，而且在大鼠海马组织中，在 120 μm 的深部具有高双光子截面值（δ≈100 GM）。
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