Sun Jingguo, Xu Junhong, Ma Qiujuan, Mao Guojiang, Zhu Nannan, Tian Meiju, Li Linke, Liu Shuzhen
School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China.
Department of Dynamical Engineering, North China University of Water Resources and Electric Power, Zhengzhou 450011, China.
Int J Anal Chem. 2022 Feb 14;2022:7649230. doi: 10.1155/2022/7649230. eCollection 2022.
Hypochlorous acid (HOCl) was crucial for maintaining the homeostasis in cells and plays vital roles in many physiological and pathological processes. In this work, a highly selective fluorescent probe for hypochlorous acid in living cells was constructed and prepared based on a naphthalene derivative. A naphthalene derivative was utilized as the fluorescent group, and ,-dimethylthiocarbamate was applied as the selective recognition site for HOCl. Before adding HOCl, the fluorescent probe exhibited weak fluorescence. Upon adding HOCl, the fluorescent probe displayed remarkable fluorescence enhancement. The fluorescence intensity at 502 nm showed a linear response to the concentration of HOCl from 3.0 × 10 to 1.0 × 10 mol·L. The detection limit was estimated to be 1.5 × 10 mol·L for HOCl. The fluorescent probe showed fast response and outstanding selectivity toward HOCl. It owned good biocompatibility and had also been successfully applied in the confocal imaging of exogenous and endogenous HOCl in living cells.
次氯酸（HOCl）对于维持细胞内稳态至关重要，并且在许多生理和病理过程中发挥着关键作用。在本研究中，基于萘衍生物构建并制备了一种用于活细胞中次氯酸的高选择性荧光探针。以萘衍生物作为荧光基团，以N，N-二甲基硫代氨基甲酸盐作为HOCl的选择性识别位点。在加入HOCl之前，荧光探针表现出微弱的荧光。加入HOCl后，荧光探针显示出显著的荧光增强。502nm处的荧光强度对HOCl浓度在3.0×10⁻⁷至1.0×10⁻⁵mol·L⁻¹范围内呈线性响应。HOCl的检测限估计为1.5×10⁻⁷mol·L⁻¹。该荧光探针对HOCl表现出快速响应和出色的选择性。它具有良好的生物相容性，并且已成功应用于活细胞中外源和内源HOCl的共聚焦成像。
