a Department of Medical Biotechnology, Faculty of Advanced Medical Sciences , Tabriz University of Medical Sciences , Tabriz , Iran.
b Stem Cell Research Center , Tabriz University of Medical Sciences , Tabriz , Iran.
Artif Cells Nanomed Biotechnol. 2018 Jun;46(4):819-830. doi: 10.1080/21691401.2017.1345925. Epub 2017 Jul 11.
The present study describes the effects of Watercress extract (WE) based electrospun nanofibrous mats on the regulation of adhesion, proliferation, cytoprotection and stemness preservation of adipose-derived stem cells (ADSCs). Watercress (Nasturtium officinale) is one of the most important medicinal plant with a board spectrum of biological functions. For this purpose, WE-loaded PCL-PEG nanofibers were fabricated by electrospinning and characterized using FE-SEM and FTIR. Adhesion, proliferation and cytoprotection of ADSCs on the nanofibers was investigated using FE-SEM and MTT assays. Analysis of cell cycle was carried out by flow-cytometry. Finally, qPCR was applied to measure the expression levels of cell cycle-regulated genes and stemness markers of ADSCs grown on the nanofibers. In this study, we found that WE-loaded PCL-PEG nanofibers had great antioxidant potential and exhibited higher cytoprotection, better adhesion, and significantly increased proliferation of ADSCs. The greater proliferation and preserving stemness ability of ADSCs on WE based nanofibers was further confirmed by higher expression levels of cell cycle-regulated genes and stemness markers. These results demonstrate that WE-loaded PCL-PEG electrospun nanofibrous mats appear suitable to support ADSCs adhesion and proliferation while concurrently preserving the cell stemness, therefore representing a hopeful approach for applying in stem cell based regenerative medicine.
本研究描述了西洋菜提取物（WE）负载的电纺纳米纤维垫对脂肪来源干细胞（ADSCs）的黏附、增殖、细胞保护和干性保存的调节作用。西洋菜（Nasturtium officinale）是一种最重要的药用植物之一，具有广泛的生物学功能。为此，通过静电纺丝制备了 WE 负载的 PCL-PEG 纳米纤维，并通过 FE-SEM 和 FTIR 进行了表征。使用 FE-SEM 和 MTT 分析评估了 ADSCs 在纳米纤维上的黏附、增殖和细胞保护作用。通过流式细胞术进行细胞周期分析。最后，通过 qPCR 测量了在纳米纤维上生长的 ADSCs 的细胞周期调控基因和干性标记物的表达水平。在这项研究中，我们发现 WE 负载的 PCL-PEG 纳米纤维具有很强的抗氧化潜力，表现出更高的细胞保护作用、更好的黏附性，并显著增加了 ADSCs 的增殖。ADSCs 在 WE 负载的纳米纤维上表现出更高的增殖和保持干性能力，这进一步得到了细胞周期调控基因和干性标记物表达水平更高的证实。这些结果表明，WE 负载的 PCL-PEG 电纺纳米纤维垫适合支持 ADSCs 的黏附和增殖，同时保持细胞干性，因此代表了一种有希望的应用于基于干细胞的再生医学的方法。
