Alino S F, Garcia D, Uvnäs-Moberg K
Acta Physiol Scand. 1986 Jan;126(1):1-8. doi: 10.1111/j.1748-1716.1986.tb07780.x.
The object of the present work was to study the influence of antral pH, of aspirin and indomethacin, and of prostaglandins A2, E1 and E2 on the intragastric output of somatostatin and gastrin induced by electrical vagal stimulation (5 V, 2 ms, 2, 5 and 10 Hz). Experiments were performed on anaesthetized rats in which the stomach was perfused with a dextran solution (pH approximately 1.5 to approximately 6). To assess the effect of vagal stimulations on gastric acid secretion and on gastrin and somatostatin release, pH as well as somatostatin and gastrin levels was recorded in the perfusate effluent. During perfusion of the stomach with dextran solution, pH approximately 6, vagal stimulation at 10 Hz decreased perfusate pH to 1.3 and gastrin and somatostatin were released at a ratio of 2:1. Less gastrin and more somatostatin (ratio 0.1:1) was released by the same stimulation when the stomach was pre-perfused with acid solution (pH approximately 1.5), although also in these experiments the pH of the perfusate fell to 1.3. When vagal stimulations were performed after the rats had been pretreated with aspirin or indomethacin, more somatostatin than gastrin was detected in the perfusate independently of the perfusate pH. The vagally induced intraluminal release of somatostatin occurring in aspirin-treated animals was abolished by a low dose of atropine (0.05 mg kg-1) or by a simultaneous infusion of prostaglandin E1 (30 micrograms kg-1 h-1).
本研究的目的是探讨胃窦pH值、阿司匹林和吲哚美辛，以及前列腺素A2、E1和E2对电刺激迷走神经（5V，2ms，2、5和10Hz）诱导的胃内生长抑素和胃泌素分泌的影响。实验在麻醉大鼠身上进行，胃用葡聚糖溶液（pH约为1.5至约6）灌注。为了评估迷走神经刺激对胃酸分泌以及胃泌素和生长抑素释放的影响，记录灌流液流出物中的pH值以及生长抑素和胃泌素水平。在用pH约为6的葡聚糖溶液灌注胃期间，10Hz的迷走神经刺激使灌流液pH值降至1.3，胃泌素和生长抑素以2:1的比例释放。当胃预先用酸性溶液（pH约为1.5）灌注时，相同刺激释放的胃泌素较少，生长抑素较多（比例为0.1:1），尽管在这些实验中灌流液的pH值也降至1.3。当大鼠用阿司匹林或吲哚美辛预处理后进行迷走神经刺激时，灌流液中检测到的生长抑素比胃泌素多，与灌流液pH值无关。低剂量阿托品（0.05mg kg-1）或同时输注前列腺素E1（30μg kg-1 h-1）可消除阿司匹林处理动物中迷走神经诱导的腔内生长抑素释放。
