Zhao Xiangsheng, Wei Jianhe, Zhou Yakui, Kong Weijun, Yang Meihua
Hainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Haikou, 571100, China.
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193, China.
AMB Express. 2017 Dec;7(1):151. doi: 10.1186/s13568-017-0450-x. Epub 2017 Jul 11.
In the storage of Alpinia oxyphylla, growth of mildew (especially toxic fungi, such as Aspergillus flavus) is a potential safety risk. Few reports have investigated how A. oxyphylla storage conditions impact mold growth or how mold growth impacts the bioactive components of A. oxyphylla. In this study, sterilized A. oxyphylla samples were contaminated by artificial inoculation of A. flavus spores. The main chemical components and aflatoxin levels in the infected A. oxyphylla samples were characterized. Central composite design-response surface methodology was used to study the effects of different temperature and humidity of storage conditions on the fungal growth in A. oxyphylla and accumulation of aflatoxins. The results showed that aflatoxins levels can be minimized by storing samples at temperatures below 25 °C and with humidity less than 85%. Additionally, we found that the yield and composition of volatile oil in A. oxyphylla exhibited small changes due to mold growth. However, polysaccharide content reduced remarkably. Temperatures below 25 °C and humidity below 85% were the best storage conditions to preventing A. oxyphylla from becoming moldy. Our results provide the theoretical basis for future studies the effects of storage conditions and mold growth on the quality and safety of traditional Chinese medicines (TCMs).
在益智储存过程中，发霉（尤其是有毒真菌，如黄曲霉）的生长是一个潜在的安全风险。很少有报告研究益智的储存条件如何影响霉菌生长，或者霉菌生长如何影响益智的生物活性成分。在本研究中，通过人工接种黄曲霉孢子对灭菌后的益智样品进行污染。对感染后的益智样品中的主要化学成分和黄曲霉毒素水平进行了表征。采用中心复合设计-响应面法研究了不同温度和湿度的储存条件对益智中真菌生长和黄曲霉毒素积累的影响。结果表明，将样品储存在25℃以下、湿度低于85%的条件下，黄曲霉毒素水平可降至最低。此外，我们发现益智中挥发油的产量和成分因霉菌生长而变化较小。然而，多糖含量显著降低。25℃以下的温度和85%以下的湿度是防止益智发霉的最佳储存条件。我们的结果为今后研究储存条件和霉菌生长对中药质量和安全性的影响提供了理论依据。
