Estrada Efrén, Rivera Del Álamo Maria M, Rodríguez-Gil Joan E, Yeste Marc
Unit of Animal Reproduction, Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Autonomous University of Barcelona, E-08193 Bellaterra (Cerdanyola del Vallès), Barcelona, Spain.
Unit of Animal Reproduction, Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Autonomous University of Barcelona, E-08193 Bellaterra (Cerdanyola del Vallès), Barcelona, Spain.
Cryobiology. 2017 Oct;78:56-64. doi: 10.1016/j.cryobiol.2017.07.002. Epub 2017 Jul 8.
Adding cryopreservation media with reduced glutathione (GSH) has previously been shown to maintain the motility, membrane integrity and fertilizing ability of frozen-thawed boar sperm, although the effects of GSH on good (GFE) and poor freezability (PFE) ejaculates rely upon the intrinsic ejaculate freezability. The resilience to withstand freeze-thawing procedures has previously been related to the existence of a specific distribution of motile sperm subpopulations, which differs between GFE and PFE. Thus, the main aim of this study was to determine whether the addition of GSH to freezing media has any impact on the distribution of motile sperm subpopulations in GFE and PFE. With this purpose, 18 GFE and 13 PFE were cryopreserved with or without 2 mM GSH. Sperm quality and motile subpopulations were evaluated at 30 min and 4 h post-thawing. Three subpopulations were identified and the percentages of spermatozoa belonging to the fastest and most linear subpopulation, which was referred as 'SP1', decreased over post-thawing time. Good freezability ejaculates that were cryopreserved in the presence of 2 mM exhibited a significantly higher percentage of spermatozoa belonging to SP1 than the other combinations of treatment and freezability both at 30 min (mean ± SEM: GFE-C: 16.6 ± 0.4; GFE-GSH 27.7 ± 0.6) and 4 h post-thawing (GFE-C: 7.8 ± 0.2 vs.
GFE-GSH: 16.7 ± 0.4). In conclusion, the positive effect of GSH on the motility of frozen-thawed sperm is related to a specific sperm subpopulation (SP1), which could coincide with the fertile sperm one.
先前已表明添加含还原型谷胱甘肽（GSH）的冷冻保存培养基可维持冻融后公猪精子的活力、膜完整性和受精能力，尽管GSH对优质（GFE）和冷冻性差（PFE）的射精的影响取决于射精固有的冷冻性。先前已表明，抵抗冻融程序的能力与活动精子亚群的特定分布有关，GFE和PFE之间存在差异。因此，本研究的主要目的是确定在冷冻培养基中添加GSH是否对GFE和PFE中活动精子亚群的分布有任何影响。为此，将18份GFE和13份PFE在有或无2 mM GSH的情况下进行冷冻保存。在解冻后30分钟和4小时评估精子质量和活动亚群。鉴定出三个亚群，属于最快和最线性亚群（称为“SP1”）的精子百分比在解冻后时间内下降。在2 mM GSH存在下冷冻保存的优质冷冻性射精在解冻后30分钟（平均值±标准误：GFE-C：16.6±0.4；GFE-GSH 27.7±0.6）和4小时时，属于SP1的精子百分比均显著高于其他处理和冷冻性组合（GFE-C：7.8±0.2对GFE-GSH：16.7±0.4）。总之，GSH对冻融精子活力的积极作用与特定的精子亚群（SP1）有关，该亚群可能与可育精子亚群一致。
