Etaee Farshid, Asadbegi Masoumeh, Taslimi Zahra, Shahidi Siamak, Sarihi Abdolrahman, Soleimani Asl Sara, Komaki Alireza
Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran; Rahe Sabz Addiction Rehabilitation Clinic, Hamadan University of Medical Sciences, Hamadan, Iran.
Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
Neurosci Lett. 2017 Aug 10;655:172-178. doi: 10.1016/j.neulet.2017.04.043. Epub 2017 Jul 8.
Methamphetamine (Meth) abuse and dependence are major global problems. Most of previous studies showed that Meth is anxiogenic. While buprenorphine (Bup) is used to treat anxiety-related behaviors, the effects of Meth in combination with Bup on anxiety-like behavior are unclear. In this study, we examined the effects of these drugs on anxiety-like behavior with the elevated plus maze (EPM) and open field (OF) tests, which are widely used to assess anxiety-like behavior in small rodents. Forty male Wistar rats were divided into four groups: sham, Meth, Bup, and Bup+Meth. The groups were administered their assigned treatments for 7days. The time spent in the open arms, and number of total entries into the arms (total activity) in the EPM were recorded. In addition, locomotor activity and number of entrances into the center area in the OF were recorded. The 7-day administration of Meth or Bup increased open arm exploration in the EPM. In contrast, the combined administration of Bup and Meth had the opposite effects. In addition, Meth and Bup had no effects on total and locomotor activity. Furthermore, the rats in the Meth and Bup groups spent more time in the center of the OF, while the group given both Bup and Meth spent less time in the center of the OF. The administration of Meth and Bup alone was anxiolytic in rats, whereas the coadministration of Bup and Meth was anxiogenic.
甲基苯丙胺（冰毒）滥用和成瘾是全球性的重大问题。以往大多数研究表明，冰毒具有致焦虑作用。虽然丁丙诺啡（丁丙诺啡）用于治疗与焦虑相关的行为，但冰毒与丁丙诺啡联合使用对焦虑样行为的影响尚不清楚。在本研究中，我们通过高架十字迷宫（EPM）和旷场试验（OF）来检测这些药物对焦虑样行为的影响，这两种试验被广泛用于评估小型啮齿动物的焦虑样行为。40只雄性Wistar大鼠被分为四组：假手术组、冰毒组、丁丙诺啡组和丁丙诺啡+冰毒组。各组接受指定治疗7天。记录大鼠在EPM中在开放臂停留的时间以及进入臂的总次数（总活动量）。此外，记录大鼠在OF中的运动活动和进入中心区域的次数。冰毒或丁丙诺啡给药7天增加了大鼠在EPM中对开放臂的探索。相比之下，丁丙诺啡和冰毒联合给药则产生相反的效果。此外，冰毒和丁丙诺啡对总活动量和运动活动没有影响。此外，冰毒组和丁丙诺啡组的大鼠在OF中心区域停留的时间更长，而同时给予丁丙诺啡和冰毒的组在OF中心区域停留的时间更短。单独给予冰毒和丁丙诺啡对大鼠有抗焦虑作用，而丁丙诺啡和冰毒联合给药则有致焦虑作用。
