Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, Via P. Bucci, cubo 17/C I-87030 Rende (CS). Italy.
Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, Via P. Bucci, cubo 17/C, 87030 Rende (CS). Italy.
Curr Pharm Des. 2017;23(26):3858-3870. doi: 10.2174/1381612823666170710161519.
Current research in neural tissue-engineering is focused on the development of advanced biomaterials for the creation of sophisticated neuro-tissue analogues, showing that mimicking the in vivo tissue disposition and functions is a useful tool for the study of brain-related issues in normal and pathological states. In addition, the most common approach for developing new drug therapies is to carry out in vitro investigation before in vivo test, thus, it is increasingly important to develop valuable models that can predict the results of in vivo studies. This review presents the recent state of the art concerning the multifunctional role of biohybrid membrane systems in neuronal tissue engineering as innovative in vitro platforms with a well-controlled microenvironment, that enhance nervous system repair by guiding neuronal growth and differentiation. In vitro membrane-based models of brain tissue, created by combining neurons, membranes and therapeutic molecules, were described highlighting the innovative approaches directed to investigate specific biological phenomena as well as for testing biopharmaceutical compounds in neurodegenerative diseases, and drug delivery to the CNS. Furthermore, several examples of in vivo application of membrane-based stem cell delivery approaches for nerve regeneration were summarized.
目前，神经组织工程学的研究重点是开发先进的生物材料，以创建复杂的神经组织模拟物，研究表明，模拟体内组织的位置和功能是研究正常和病理状态下与大脑相关问题的有用工具。此外，开发新的药物疗法的最常见方法是在进行体内试验之前进行体外研究，因此，开发能够预测体内研究结果的有价值的模型变得越来越重要。本文综述了生物杂交膜系统在神经元组织工程中的多功能作用的最新研究进展，该系统作为具有良好控制的微环境的创新体外平台，通过引导神经元的生长和分化来增强神经系统的修复。本文通过结合神经元、膜和治疗分子，描述了脑组织的基于膜的体外模型，突出了针对特定生物现象进行研究以及用于测试神经退行性疾病中的生物制药化合物和向中枢神经系统递药的创新方法。此外，还总结了基于膜的干细胞递药方法在神经再生中的一些体内应用实例。
