Kawamura Yoshiki, Ohye Tamae, Miura Hiroki, Ihira Masaru, Kato Yuri, Kurahashi Hiroki, Yoshikawa Tetsushi
Department of Pediatrics, Fujita Health University School of Medicine, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi 470-1192, Japan.
Present address: Division of Viral Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, Food and Drug Administration, 1090340 New Hampshire Avenue, Silver Spring, MD 20993, USA.
J Gen Virol. 2017 Jul;98(7):1823-1830. doi: 10.1099/jgv.0.000834. Epub 2017 Jul 12.
Integration of the complete human herpesvirus 6 (HHV-6) genome into the telomere of a chromosome has been reported in some individuals (inherited chromosomally integrated HHV-6; iciHHV-6). Since the proportion of iciHHV-6-positive individuals with integration in chromosome 22 is high in Japan, we hypothesized a founder effect. In this study, we sought to elucidate the reason for the high proportion of viral integrations into chromosome 22. We analyzed six cases of iciHHV-6A and two cases of iciHHV-6B, including one iciHHV-6A case with a matched sample from a father and one iciHHV-6B case with a matched sample from a mother. In iciHHV-6A, the same copy numbers of viral telomeric repeat sequences (TRS) and the same five microsatellite markers were detected in both the index case and paternal sample. Moreover, the same five microsatellite markers were demonstrated in four cases and the same copy numbers of viral TRS were demonstrated in two pairs of two cases. The present microsatellite analysis suggested that the viral genomes detected in some iciHHV-6A patients were derived from a common ancestral integration.
据报道，在一些个体中，完整的人类疱疹病毒6型（HHV-6）基因组已整合到染色体的端粒中（遗传性染色体整合HHV-6；iciHHV-6）。由于在日本，染色体22上存在整合的iciHHV-6阳性个体比例较高，我们推测存在奠基者效应。在本研究中，我们试图阐明病毒整合到染色体22比例较高的原因。我们分析了6例iciHHV-6A和2例iciHHV-6B，其中包括1例iciHHV-6A病例及其父亲的匹配样本，以及1例iciHHV-6B病例及其母亲的匹配样本。在iciHHV-6A中，在索引病例和父本样本中检测到相同拷贝数的病毒端粒重复序列（TRS）和相同的五个微卫星标记。此外，在4例中显示了相同的五个微卫星标记，在两对两例中显示了相同拷贝数的病毒TRS。目前的微卫星分析表明，在一些iciHHV-6A患者中检测到的病毒基因组源自共同的祖先整合。
