MicroRNA-613 通过靶向 CDK9 抑制胃癌的进展。
MicroRNA-613 inhibits the progression of gastric cancer by targeting CDK9.
机构信息
a Department of General Surgery , Xiangya Hospital, Central South University , Changsha , China.
b Department of Pharmacy , Xiangya Hospital, Central South University , Changsha , China.
出版信息
Artif Cells Nanomed Biotechnol. 2018 Aug;46(5):980-984. doi: 10.1080/21691401.2017.1351983. Epub 2017 Jul 13.
MicroRNAs (miRNAs) play an important role in the development and progression of human malignancy. miR-613, as a tumour suppressor, was reported to decrease in several tumours. However, the expression levels and role of miR-613 in gastric cancer remain unknown. In this study, we found that miR-613 was evidently downregulated in gastric cancer tissue and cell. The functional analysis showed that miR-613 suppressed cell proliferation and migration in gastric cancer. Next, the dual-luciferase reporter system supported CDK9 as a direct target gene of miR-613. miR-613 mimics evidently repressed CDK9 expression in gastric cancer cells. Furthermore, we found that CDK9 in upregulated in gastric cancer and the CDK9 expression levels were inversely correlated with that of miR-613 in gastric cancer tissues. Overall, the results revealed that miR-613, as a tumour suppressor, involves in gastric cancer progression and metastasis by targeting CDK9, implying a novel potential therapeutic target for the treatment of gastric cancer.
微小 RNA（miRNAs）在人类恶性肿瘤的发生和发展中起着重要作用。miR-613 作为一种肿瘤抑制因子，据报道在几种肿瘤中减少。然而，miR-613 在胃癌中的表达水平和作用仍不清楚。在本研究中，我们发现 miR-613 在胃癌组织和细胞中明显下调。功能分析表明，miR-613 抑制胃癌细胞的增殖和迁移。接下来，双荧光素酶报告系统支持 CDK9 是 miR-613 的直接靶基因。miR-613 模拟物在胃癌细胞中明显抑制 CDK9 的表达。此外，我们发现 CDK9 在胃癌中上调，并且 CDK9 的表达水平与胃癌组织中 miR-613 的表达水平呈负相关。总的来说，这些结果表明，miR-613 通过靶向 CDK9 参与胃癌的进展和转移，为胃癌的治疗提供了一个新的潜在治疗靶点。
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