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免疫金电子显微镜分析揭示了拟南芥根尖细胞中对布雷菲德菌素A敏感的生长素簇。

Immunogold-EM analysis reveal brefeldin a-sensitive clusters of auxin in Arabidopsis root apex cells.

作者信息

Mettbach U, Strnad M, Mancuso S, Baluška F

机构信息

IZMB, University of Bonn, Bonn, Germany.

Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany AS CR & Palacký University, Olomouc, Czech Republic.

出版信息

Commun Integr Biol. 2017 May 9;10(3):e1327105. doi: 10.1080/19420889.2017.1327105. eCollection 2017.

DOI:10.1080/19420889.2017.1327105
PMID:28702129
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5501221/
Abstract

Immunogold electron microscopy (EM) study of Arabidopsis root apices analyzed using specific IAA antibody and high-pressure freeze fixation technique allowed, for the first time, vizualization of subcellular localization of IAA in cells assembled intactly within plant tissues. Our quantitative analysis reveals that there is considerable portion of IAA gold particles that clusters within vesicles and membraneous compartments in all root apex cells. There are clear tissue-specific and developmental differences of clustered IAA in root apices. These findings have significant consequences for our understanding of this small molecule which is controlling plant growth, development and behavior.

摘要

利用特异性吲哚-3-乙酸（IAA）抗体和高压冷冻固定技术对拟南芥根尖进行免疫金电子显微镜（EM）研究，首次实现了在植物组织内完整组装的细胞中可视化IAA的亚细胞定位。我们的定量分析表明，在所有根尖细胞中，相当一部分IAA金颗粒聚集在囊泡和膜性区室中。根尖中聚集的IAA存在明显的组织特异性和发育差异。这些发现对于我们理解这种控制植物生长、发育和行为的小分子具有重要意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ada0/5501221/da3a6aeda26d/kcib-10-03-1327105-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ada0/5501221/bdee990e3648/kcib-10-03-1327105-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ada0/5501221/2cb540e93a53/kcib-10-03-1327105-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ada0/5501221/4183588f16b1/kcib-10-03-1327105-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ada0/5501221/da3a6aeda26d/kcib-10-03-1327105-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ada0/5501221/bdee990e3648/kcib-10-03-1327105-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ada0/5501221/2cb540e93a53/kcib-10-03-1327105-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ada0/5501221/4183588f16b1/kcib-10-03-1327105-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ada0/5501221/da3a6aeda26d/kcib-10-03-1327105-g004.jpg

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The PLETHORA Gene Regulatory Network Guides Growth and Cell Differentiation in Arabidopsis Roots.多基因调控网络指导拟南芥根的生长和细胞分化。
质膜通道在胚胎发生早期调节生长素依赖基因中是否起主要作用？
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Stress-Induced Microspore Embryogenesis Requires Endogenous Auxin Synthesis and Polar Transport in Barley.胁迫诱导的大麦小孢子胚胎发生需要内源性生长素的合成和极性运输。
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