如何加快聚合酶链式反应
How to speed up the polymerase chain reaction.
作者信息
Bustin Stephen A
机构信息
Faculty of Medical Science, Postgraduate Medical Institute, Anglia Ruskin University, Chelmsford CM1 1SQ, UK.
出版信息
Biomol Detect Quantif. 2017 Jun 20;12:10-14. doi: 10.1016/j.bdq.2017.05.002. eCollection 2017 Jun.
Abstract
Reducing the time taken to run qPCR assays on today's qPCR cyclers is rather straightforward and requires no specialised reagents or instruments. As the first article in a new series of short technical reports, I demonstrate that it is possible to reduce significantly both denaturation temperatures and cycling times, whilst retaining sensitivity and specificity of the original qPCR conditions.
摘要
在当今的定量聚合酶链反应（qPCR）仪上缩短运行qPCR检测所需的时间相当简单，且不需要特殊的试剂或仪器。作为新系列简短技术报告中的第一篇文章，我证明了在保持原始qPCR条件的灵敏度和特异性的同时，显著降低变性温度和循环次数是可行的。
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