Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu, Kagawa, Japan.
Biosens Bioelectron. 2011 Sep 15;27(1):88-94. doi: 10.1016/j.bios.2011.06.022. Epub 2011 Jun 26.
A novel flow-through polymerase chain reaction (PCR) microfluidic system using vapor pressure was developed that can achieve ultra-rapid, small-volume DNA amplification on a chip. The 40-cycle amplification can be completed in as little as 120 s, making this device the fastest PCR system in the world. The chip device is made of a pressure-sensitive polyolefin (PSP) film and cyclo-olefin polymer (COP) substrate which was processed by cutting-work to fabricate the microchannel. The enclosed structure of the microchannel was fabricated solely by weighing the PSP film on the COP substrate, resulting in superior practical application. The vapor pressure in the denaturation zone of the destabilizing flow source was applied to the flow force, and ultra-rapid, efficient amplification was accomplished with a minimal amount of PCR reagents for detection. The flowing rhythm created by vapor pressure minimized the residual PCR products, leading to highly efficient amplification. For field test analysis, airborne dust was collected from a public place and tested for the presence of anthrax. The PCR chip had sufficient sensitivity for anthrax identification. The fastest time from aerosol sampling to detection was theoretically estimated as 8 min.
一种新型的采用蒸气压的连续流聚合酶链反应（PCR）微流控系统，可实现在芯片上进行超快速、小体积的 DNA 扩增。40 个循环的扩增可以在 120 秒内完成，这使得该设备成为世界上最快的 PCR 系统。该芯片装置由压敏聚烯烃（PSP）薄膜和环烯烃聚合物（COP）基底制成，通过切割工艺制造微通道。微通道的封闭结构仅通过在 COP 基底上称重 PSP 薄膜来制造，从而具有出色的实际应用。非稳定流源的变性区中的蒸气压被应用于流动力，从而以最小量的 PCR 试剂进行检测，实现了超快速、高效的扩增。蒸气压产生的流动节奏最大限度地减少了残留的 PCR 产物，从而实现了高效扩增。为了进行现场测试分析，从公共场所采集了空气中的尘埃，并对炭疽进行了检测。PCR 芯片具有足够的炭疽鉴定灵敏度。从气溶胶采样到检测的最快时间理论上估计为 8 分钟。
