Kaldany R R, Campanelli J T, Schaefer M, Shyamala M, Scheller R H
Peptides. 1985;6 Suppl 3:445-9. doi: 10.1016/0196-9781(85)90412-7.
The proteins of identified cells from the Aplysia californica central nervous system were labeled with radioactive amino acids and fractionated on SDS acrylamide gels containing 6 M urea. Most of the large cells contain prominent, cell-specific protein products in the molecular weight range between 3 and 30 KD. The molecular weights of the largest specific prevalent protein products are in good agreement with the predicted molecular weights of precursors as determined from an analysis of cDNA clones homologous to mRNA's specifically expressed in several of these neurons. Biologically active peptides have been found in many of these cells. These data, and other indirect evidence suggests that the synthesis of a large amount of a particular protein in this molecular weight range is indicative of the synthesis of a neurosecretory product. We conclude that most, if not all, large neurons in the Aplysia central nervous system are peptidergic.
用放射性氨基酸标记加利福尼亚海兔中枢神经系统中已鉴定细胞的蛋白质，并在含有6M尿素的SDS聚丙烯酰胺凝胶上进行分级分离。大多数大细胞在分子量范围为3至30KD内含有显著的、细胞特异性的蛋白质产物。最大的特异性普遍存在的蛋白质产物的分子量与通过对与这些神经元中几种特异性表达的mRNA同源的cDNA克隆分析所确定的前体预测分子量高度一致。在许多这些细胞中都发现了生物活性肽。这些数据以及其他间接证据表明，在这个分子量范围内大量特定蛋白质的合成表明神经分泌产物的合成。我们得出结论，加利福尼亚海兔中枢神经系统中的大多数（如果不是全部）大神经元是肽能神经元。
