Lipok Christian, Hippler Jörg, Schmitz Oliver J
University of Duisburg-Essen, Applied Analytical Chemistry, Universitaetsstr. 5, 45141 Essen, Germany.
University of Duisburg-Essen, Applied Analytical Chemistry, Universitaetsstr. 5, 45141 Essen, Germany.
J Chromatogr A. 2018 Feb 9;1536:50-57. doi: 10.1016/j.chroma.2017.07.013. Epub 2017 Jul 8.
A two-dimensional GC (2D-GC) method was developed and coupled to an ion mobility-high resolution mass spectrometer, which enables the separation of complex samples in four dimensions (2D-GC, ion mobilility spectrometry and mass spectrometry). This approach works as a continuous multiheart-cutting GC-system (GC+GC), using a long modulation time of 20s, which allows the complete transfer of most of the first dimension peaks to the second dimension column without fractionation, in comparison to comprehensive two-dimensional gas chromatography (GCxGC). Hence, each compound delivers only one peak in the second dimension, which simplifies the data handling even when ion mobility spectrometry as a third and mass spectrometry as a fourth dimension are introduced. The analysis of a plant extract from Calendula officinales shows the separation power of this four dimensional separation method. The introduction of ion mobility spectrometry provides an additional separation dimension and allows to determine collision cross sections (CCS) of the analytes as a further physicochemical constant supporting the identification. A CCS database with more than 800 standard substances including drug-like compounds and pesticides was used for CCS data base search in this work.
开发了一种二维气相色谱（2D-GC）方法，并将其与离子淌度-高分辨率质谱仪联用，该方法能够在四个维度（二维气相色谱、离子淌度光谱和质谱）上分离复杂样品。这种方法作为一种连续的多中心切割气相色谱系统（GC+GC）运行，使用20秒的长调制时间，与全二维气相色谱（GCxGC）相比，这允许将大部分第一维度的峰完整地转移到第二维度的色谱柱上而不进行分馏。因此，每种化合物在第二维度仅产生一个峰，即使引入作为第三维度的离子淌度光谱和作为第四维度的质谱，这也简化了数据处理。对金盏花植物提取物的分析显示了这种四维分离方法的分离能力。离子淌度光谱的引入提供了一个额外的分离维度，并允许确定分析物的碰撞截面（CCS），作为支持鉴定的另一个物理化学常数。在这项工作中，使用了一个包含800多种标准物质（包括类药物化合物和农药）的CCS数据库进行CCS数据库搜索。
