Kawasaki Hiroshi, Kretsinger Robert H
Department of Medical Life Science, Graduate School of Medical Life Science, Yokohama City University, Yokohama, Kanagawa, 230-0045, Japan.
Department of Biology, University of Virginia, Charlottesville, Virgina, 22904.
Protein Sci. 2017 Oct;26(10):1898-1920. doi: 10.1002/pro.3233. Epub 2017 Jul 27.
We have classified 865 sequences of EF-hand proteins from five proteomes into 156 subfamilies. These subfamilies were put into six groups. Evolutionary relationships among subfamilies and groups were analyzed from the inferred ancestral sequence for each subfamily. CTER, CPV, and PEF groups arose from a common EF-lobe (pair of adjacent EF-hands). They have two or more EF-lobes; the relative positions of their EF-lobes differ from each other. Comparisons of the ancestral sequences and the inferred structures of the EF-lobes of these groups indicate that the mutual positions of EF-lobes were established soon after divergence of an EF-lobe for each group and before the duplication and fusion of EF-lobe gene(s). These ancestral sequences reveal that some subfamilies in low similarity and isolated groups did not evolve from the EF-lobe precursor, even if their conformations are similar to the canonical EF-hand. This is an example of convergent evolution.
我们已将来自五个蛋白质组的865个EF-手型蛋白序列分类为156个亚家族。这些亚家族被分为六组。从每个亚家族推断的祖先序列分析亚家族和组之间的进化关系。CTER、CPV和PEF组起源于一个共同的EF叶（一对相邻的EF-手）。它们有两个或更多的EF叶；它们的EF叶的相对位置彼此不同。这些组的祖先序列和推断的EF叶结构的比较表明，EF叶的相互位置在每组的一个EF叶分化后不久且在EF叶基因复制和融合之前就已确立。这些祖先序列表明，一些相似度低且孤立的组中的亚家族并非从EF叶前体进化而来，即使它们的构象与典型的EF-手相似。这是趋同进化的一个例子。
