Shirinzadeh Haji, Yazdanpanah Abolfazl, Karponis Dimitrios, Aghabarari Behzad, Tahmasbi Mohammad, Seifalian Alexander, Mozafari Masoud
Semiconductor Department, Materials and Energy Research Center (MERC), P.O. Box 14155-4777, Tehran, Iran.
Biomaterials Group, Faculty of Biomedical Engineering (Center of Excellence), Amirkabir University of Technology, Tehran, Iran.
Recent Pat Biotechnol. 2018;12(2):126-133. doi: 10.2174/1872208311666170713150220.
Glucose sensors have been extensively researched in patent studies and manufactured a tool for clinical diabetes diagnosis. Although some kinds of electrochemical enzymatic glucose sensors have been commercially successful, there is still room for improvement, in selectivity and reliability of these sensors. Because of the intrinsic disadvantages of enzymes, such as high fabrication cost and poor stability, non-enzymatic glucose sensors have recently been promoted as next generation diagnostic tool due to their relatively low cost, high stability, prompt response, and accuracy.
In this research, a novel free standing and binder free non-enzymatic electrochemical sensor was manufactured using in situ grown copper (Cu) and cobalt (Co) on a silicon (Si) substrate.
Scanning High-Energy Electron Diffraction (SHEED) and Edward deposition methods were used to synthesise the sensors.
Morphological observations showed that Cu and Co homogeneously formed nanorod-like shapes over the Si substrate. The elemental composition and structure of the prepared sensors were identified by Reflection High-Energy Electron Diffraction (RHEED). In terms of electrochemical properties, for the enzyme-free glucose sensor, voltammograms showed that the peak currents increased when the glucose solution was injected into the electrolytic cell. The electrical relation of voltage versus current was linear, as shown in the experimental data. Another effective parameter changed the magnetic field; and the linear behaviour of the electrical resistance of Co remained unaltered.
In the optimum annealing temperature, where the magnetic field increased, the properties of the samples remained constant. In other words, in the selected annealing temperature, resistance and stability of the layers increased in a significant manner.
葡萄糖传感器在专利研究中已得到广泛研究，并成为临床糖尿病诊断的一种工具。尽管某些类型的电化学酶葡萄糖传感器已在商业上取得成功，但这些传感器在选择性和可靠性方面仍有改进空间。由于酶存在诸如制造成本高和稳定性差等固有缺点，非酶葡萄糖传感器因其相对低成本、高稳定性、快速响应和准确性，最近被推广为下一代诊断工具。
在本研究中，通过在硅（Si）衬底上原位生长铜（Cu）和钴（Co），制造了一种新型的独立且无粘合剂的非酶电化学传感器。
使用扫描高能电子衍射（SHEED）和爱德华兹沉积方法合成传感器。
形态学观察表明，Cu和Co在Si衬底上均匀形成纳米棒状形状。通过反射高能电子衍射（RHEED）确定所制备传感器的元素组成和结构。就电化学性质而言，对于无酶葡萄糖传感器，伏安图表明当将葡萄糖溶液注入电解池时峰值电流增加。电压与电流的电关系呈线性，如实验数据所示。另一个有效参数改变了磁场；并且Co的电阻的线性行为保持不变。
在最佳退火温度下，随着磁场增加，样品的性质保持不变。换句话说，在选定的退火温度下，层的电阻和稳定性显著增加。
