Aguilera O, Rocha I, Lopes M S, Lima I, Lopes R T, Machado A S, Guimarães R B, Crapez M A C, Tenório M C, Nepomuceno A
Departamento de Biologia Marinha, Programa de Pós-graduação em Biologia Marinha e Ambientes Costeiros, Universidade Federal Fluminense (UFF), Niterói, RJ, Brazil.
Nuclear Instrumentation Laboratory, Nuclear Engineering Program/COPPE, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
J Fish Dis. 2017 Dec;40(12):1869-1881. doi: 10.1111/jfd.12662. Epub 2017 Jul 14.
Zooarchaeological collections from shell mounds in Rio de Janeiro (2,470-4,632 cal BP) contain a high prevalence of swollen fish bones belonging to the Atlantic spadefish (Chaetodipterus faber), crevalle jack (Caranx hippos) and fat snook (Centropomus parallelus). Given the lack of knowledge of the bone degenerative process in senile fishes, this study analysed hyperostotic bone in zooarchaeological and modern specimens to obtain high-resolution morphology and microstructure reconstruction. We used microCT as well as X-ray diffraction to characterize the crystallographic changes associated with fish senility. Our results showed that trabecular microstructures in hyperostotic bones were consistent with estimated values of the per cent bone volume-to-total volume ratio (BV/TV) and were greater than 60% in cortical bone. Hyperostotic bones indicated a high radiograph density, and X-ray diffractograms showed a decrease in hydroxyapatite [Ca (PO ) (OH) ] and calcite (CaCO ) neocrystallization. These crystalline and density changes revealed an advanced stage of fish senile and indicate the vulnerability of ageing fish populations.
来自里约热内卢贝丘遗址（公元前2470 - 4632年）的动物考古学藏品中，属于大西洋鲯鳅（Chaetodipterus faber）、马鲹（Caranx hippos）和肥壮裸背电鳗（Centropomus parallelus）的鱼骨肿胀现象极为普遍。鉴于对老龄鱼类骨骼退化过程缺乏了解，本研究分析了动物考古学标本和现代标本中的骨质增生骨骼，以获得高分辨率的形态和微观结构重建。我们使用了显微CT以及X射线衍射来表征与鱼类衰老相关的晶体学变化。我们的结果表明，骨质增生骨骼中的小梁微观结构与骨体积与总体积比（BV/TV）的估计值一致，并且在皮质骨中大于60%。骨质增生骨骼显示出较高的射线照片密度，X射线衍射图显示羟基磷灰石[Ca(PO)(OH)]和方解石（CaCO）新结晶减少。这些晶体和密度变化揭示了鱼类衰老的晚期阶段，并表明老龄鱼群的脆弱性。
