Zhang Xintao, Zhang Meng, Cheng Jianli, Lv Zhaojie, Wang Feng, Cai Zhiming
Department of Urology, Shenzhen Second People's Hospital, Clinical Medicine College of Anhui Medical University, Shenzhen, Guangdong - PR China.
Graduate School of Anhui Medical University, Hefei, Anhui - P. R. China.
Int J Biol Markers. 2017 Oct 31;32(4):e454-e460. doi: 10.5301/ijbm.5000261.
Recent studies have revealed that microRNAs (miRNAs) play important roles as oncogenes or tumor suppressors in tumorigenesis and tumor development, by negatively regulating protein expression. A previous study of microarrays identified that miR-411 was down-regulated in renal cell carcinoma (RCC), while few studies investigating the role of miR-411 in the pathogenesis of RCC have been performed.
We assessed the miR-411 expression in RCC and paired adjacent normal tissues, as well as in RCC cell lines and a normal renal cell line, by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Furthermore, the effects of miR-411 on RCC and normal renal cell proliferation, apoptosis and migration were determined using MTT assay, CCK-8 assay, flow cytometry and scratch wound assay following restoration of miR-411 with synthetic mimics.
Results of qRT-PCR indicated that the expression of miR-411 was down-regulated in RCC tissues and cell lines when compared with adjacent normal tissues and a normal renal cell line. Further, results of CCK-8, MTT, cell scratch and transwell assay showed that over-expression of miR-411 suppressed RCC cell (786-O and ACHN) proliferation and migration. Flow cytometry assay revealed that miR-411 could induce RCC cell apoptosis. However, overexpression of miR-411 had no obvious effect on normal renal cell line 293T.
To sum up, miR-411 is significantly down-regulated and plays a role as a tumor suppressor in RCC. Further studies are warranted to determine the mechanisms of miR-411 in RCC pathogenesis and define the target genes of miR-411 in RCC.
最近的研究表明，微小RNA（miRNA）通过负调控蛋白质表达，在肿瘤发生和发展过程中作为癌基因或肿瘤抑制因子发挥重要作用。先前一项关于微阵列的研究发现，miR-411在肾细胞癌（RCC）中表达下调，然而，很少有研究探讨miR-411在RCC发病机制中的作用。
我们通过定量逆转录聚合酶链反应（qRT-PCR）评估了miR-411在RCC及其配对的相邻正常组织、RCC细胞系和正常肾细胞系中的表达。此外，在用合成模拟物恢复miR-411后，使用MTT法、CCK-8法、流式细胞术和划痕试验确定了miR-411对RCC和正常肾细胞增殖、凋亡和迁移的影响。
qRT-PCR结果表明，与相邻正常组织和正常肾细胞系相比，miR-411在RCC组织和细胞系中的表达下调。此外，CCK-8、MTT、细胞划痕和Transwell试验结果表明，miR-411的过表达抑制了RCC细胞（786-O和ACHN）的增殖和迁移。流式细胞术分析显示，miR-411可诱导RCC细胞凋亡。然而，miR-411的过表达对正常肾细胞系293T没有明显影响。
综上所述，miR-411在RCC中显著下调，并作为肿瘤抑制因子发挥作用。有必要进一步研究以确定miR-411在RCC发病机制中的作用机制，并确定miR-411在RCC中的靶基因。
