Pivovarenko Vasyl G, Bugera Oleksandra, Humbert Nicolas, Klymchenko Andrey S, Mély Yves
Department of Chemistry, National Taras Shevchenko, University of Kyiv, 01601, Kyiv, Ukraine.
Laboratoire de Biophotonique et Pharmacologie, UMR 7213 du CNRS, Université de Strasbourg, Faculté de Pharmacie, 74 route du Rhin, 67401, Illkirch, France.
Chemistry. 2017 Sep 4;23(49):11927-11934. doi: 10.1002/chem.201702484. Epub 2017 Aug 9.
A series of 26 3-hydroxychromones, three bis-flavonols and four 3-hydroxyquinolones were studied to evaluate their fluorescence response to interaction with ATP in buffer. The dyes differ by the total charge, the size and number of their aromatic units, as well as the position or electron-donating ability of their substituents. All of them were suggested to form complexes with ATP of 1:1 and 1:2 stoichiometry, which can be evidenced by their bright fluorescence and their 3000-6000 cm red-shifted excitation band. These fluorescent complexes allow detection of ATP concentrations over 3 orders of magnitude, whereas most other known probes cover no more than two orders. In total, the dyes allow ATP detection from 0.001 to 57 mm. In addition, most of the dye-ATP complexes can be excited in the visible and monitored in the red region of the spectrum. The response amplitude of the described dyes to ATP is as high as for the best known probes. Considering that complexation takes place at neutral pH, the studied dyes form a toolbox of fluorescent probes for intensiometric and ratiometric measurements of ATP concentration in a broad concentration range. Finally, the obtained results stimulate the idea that most of natural 3-hydroxyflavones in living cells may form complexes with ATP.
研究了一系列26种3-羟基色酮、三种双黄酮醇和四种3-羟基喹诺酮，以评估它们在缓冲液中与ATP相互作用时的荧光响应。这些染料在总电荷、芳香单元的大小和数量以及取代基的位置或给电子能力方面存在差异。所有这些染料都被认为能与ATP形成化学计量比为1:1和1:2的复合物，这可以通过它们明亮的荧光和3000 - 6000 cm的红移激发带来证明。这些荧光复合物能够检测超过3个数量级的ATP浓度，而大多数其他已知探针的检测范围不超过两个数量级。总体而言，这些染料能够检测0.001至57 mm的ATP。此外，大多数染料 - ATP复合物可以在可见光区域激发，并在光谱的红色区域进行监测。所描述的染料对ATP的响应幅度与最知名的探针一样高。考虑到络合作用在中性pH下发生，所研究的染料形成了一个荧光探针工具箱，可用于在宽浓度范围内对ATP浓度进行强度测量和比率测量。最后，所得结果引发了这样一种想法，即活细胞中大多数天然3 - 羟基黄酮可能与ATP形成复合物。
