Cho Kyungjin, Choi Minkyu, Jeong Dawoon, Lee Seockheon, Bae Hyokwan
Center for Water Resource Cycle Research, Korea Institute of Science and Technology, 39-1 Hawolgok-Dong, Seongbuk-Gu, Seoul 136-791, Republic of Korea.
Center for Water Resource Cycle Research, Korea Institute of Science and Technology, 39-1 Hawolgok-Dong, Seongbuk-Gu, Seoul 136-791, Republic of Korea; Department of Civil and Environmental Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-Gu, Seoul 120-749, Republic of Korea.
Chemosphere. 2017 Oct;185:394-402. doi: 10.1016/j.chemosphere.2017.06.123. Epub 2017 Jul 3.
The process performance and microbial niche of anaerobic ammonia oxidation (ANAMMOX) bacteria were compared in two identical bioreactors inoculated with different inoculum sources (i.e., pre-cultured ANAMMOX bacteria: PAB and activated sludge: AS) entrapped in poly(vinyl alcohol)/sodium alginate (PVA/SA) gel beads for a long-term period (i.e., 1.5 years). The start-up period with AS was longer than that with PAB; however, both bioreactors were successfully operated over the long-term with stable ANAMMOX activity. After long-term operation, the 16S rRNA gene concentration of ANAMMOX bacteria in both bioreactors was significantly increased, and thereby became comparable. In addition, Candidatus Jettenia sp. became the dominant ANAMMOX species in both bioreactors. Our results suggested that the ANAMMOX performance and microbial niche of ANAMMOX bacteria became nearly identical during long-term operation despite the use of different inoculum sources. Therefore, the use of PVA/SA gel beads entrapping AS appears to be a relevant option for constructing an ANAMMOX process in places where a full-scale ANAMMOX process has never been done previously.
在两个相同的生物反应器中，对固定在聚乙烯醇/海藻酸钠（PVA/SA）凝胶珠中的不同接种源（即预培养厌氧氨氧化细菌：PAB和活性污泥：AS）进行长期（即1.5年）的厌氧氨氧化（ANAMMOX）细菌的工艺性能和微生物生态位比较。以AS为接种源的启动期比以PAB为接种源的启动期长；然而，两个生物反应器均长期成功运行，具有稳定的厌氧氨氧化活性。长期运行后，两个生物反应器中厌氧氨氧化细菌的16S rRNA基因浓度均显著增加，从而变得相当。此外，“Candidatus Jettenia sp.”成为两个生物反应器中占主导地位的厌氧氨氧化菌属。我们的结果表明，尽管使用了不同的接种源，但在长期运行过程中，厌氧氨氧化细菌的厌氧氨氧化性能和微生物生态位几乎相同。因此，对于以前从未进行过全规模厌氧氨氧化工艺的地方，使用固定有AS的PVA/SA凝胶珠似乎是构建厌氧氨氧化工艺的一个相关选择。
