Ministry of Education Key Laboratory of Pollution Control and Ecological Remediation for Industrial Agglomeration area, College of Environment and Energy, South China University of Technology, Guangzhou, 510006, China E-mail:
Water Sci Technol. 2014;69(4):718-26. doi: 10.2166/wst.2013.762.
Anaerobic ammonium oxidation (anammox) has been proved to be a promising nitrogen removal method for treating ammonium-rich wastewater. However, because of the low-growth rate of anammox bacteria, maintenance of a sufficient amount of anammox biomass in reactor became a key factor in application. Gel immobilization is an efficient method to prevent biomass from being washed out and to promote hyper-concentrated cultures. This study focused on a nitrogen removal process by anammox enrichment culture immobilized in polyvinyl alcohol and sodium alginate (PVA-SA) gel beads. The rapid startup of reactor demonstrated that gel entrapment was supposed to be a highly effective technique for immobilizing anammox bacteria. The anammox bacteria present in the enrichment were identified to be Jettenia-like species (>98%). Moreover, the effect of hydraulic retention time (HRT), pH, and temperature on immobilized anammox processes were investigated. The effect of pH and temperature on the anammox process was evidently weakened in PVA-SA immobilized gel beads, however, the effect of HRT on the anammox reaction was enhanced. Therefore, a stable operated reactor could be obtained in an anaerobic sequencing batch reactor, which proved gel immobilization was an excellent method to maintain the biomass in anammox reactor for application.
厌氧氨氧化（anammox）已被证明是一种很有前途的处理富氨废水的脱氮方法。然而，由于厌氧氨氧化菌的生长速度较慢，在反应器中维持足够数量的厌氧氨氧化菌生物量成为应用的关键因素。凝胶固定化是防止生物量流失和促进超浓缩培养的有效方法。本研究聚焦于通过聚乙烯醇和海藻酸钠（PVA-SA）凝胶珠固定化厌氧氨氧化富集培养进行氮去除的过程。反应器的快速启动表明，凝胶包埋是固定厌氧氨氧化菌的一种非常有效的技术。富集物中的厌氧氨氧化菌被鉴定为杰滕尼亚样物种（>98%）。此外，还研究了水力停留时间（HRT）、pH 和温度对固定化厌氧氨氧化过程的影响。在 PVA-SA 固定化凝胶珠中，pH 和温度对厌氧氨氧化过程的影响明显减弱，而 HRT 对厌氧氨氧化反应的影响增强。因此，在厌氧序批式反应器中可以获得稳定运行的反应器，这证明凝胶固定化是一种维持厌氧氨氧化反应器中生物量以应用的优秀方法。
