Rodriguez Laura, Nogales Aitor, Murcia Pablo R, Parrish Colin R, Martínez-Sobrido Luis
Department of Microbiology and Immunology, University of Rochester, Rochester, NY, United States.
MRC-University of Glasgow Centre for Virus Research, Glasgow, United Kingdom.
Vaccine. 2017 Aug 3;35(34):4374-4381. doi: 10.1016/j.vaccine.2017.06.060. Epub 2017 Jul 11.
Canine influenza viruses (CIVs) cause a contagious respiratory disease in dogs. CIV subtypes include H3N8, which originated from the transfer of H3N8 equine influenza virus (EIV) to dogs; and the H3N2, which is an avian-origin virus adapted to infect dogs. Only inactivated influenza vaccines (IIVs) are currently available against the different CIV subtypes. However, the efficacy of these CIV IIVs is not optimal and improved vaccines are necessary for the efficient prevention of disease caused by CIVs in dogs. Since live-attenuated influenza vaccines (LAIVs) induce better immunogenicity and protection efficacy than IIVs, we have combined our previously described H3N8 and H3N2 CIV LAIVs to create a bivalent vaccine against both CIV subtypes. Our findings show that, in a mouse model of infection, the bivalent CIV LAIV is safe and able to induce, upon a single intranasal immunization, better protection than that induced by a bivalent CIV IIV against subsequent challenge with H3N8 or H3N2 CIVs. These protection results also correlated with the ability of the bivalent CIV LAIV to induce better humoral immune responses. This is the first description of a bivalent LAIV for the control and prevention of H3N8 and H3N2 CIV infections in dogs.
犬流感病毒（CIVs）可在犬类中引发一种传染性呼吸道疾病。CIV亚型包括源自H3N8马流感病毒（EIV）向犬类转移的H3N8；以及一种适应感染犬类的禽源病毒H3N2。目前仅有灭活流感疫苗（IIVs）可用于针对不同的CIV亚型。然而，这些CIV IIVs的效力并不理想，因此需要改进疫苗以有效预防犬类由CIVs引起的疾病。由于减毒活流感疫苗（LAIVs）比IIVs诱导出更好的免疫原性和保护效力，我们将之前描述的H3N8和H3N2 CIV LAIVs组合，制成了一种针对两种CIV亚型的二价疫苗。我们的研究结果表明，在感染小鼠模型中，二价CIV LAIV是安全的，并且在单次鼻内免疫后，能比二价CIV IIV诱导出更好的保护效果，以抵御随后H3N8或H3N2 CIVs的攻击。这些保护结果也与二价CIV LAIV诱导更好的体液免疫反应的能力相关。这是首次描述用于控制和预防犬类H3N8和H3N2 CIV感染的二价LAIV。
