由猪轮状病毒激活的小鼠骨髓来源的树突状细胞在体外刺激Th1亚型反应。
Murine bone marrow-derived DCs activated by porcine rotavirus stimulate the Th1 subtype response in vitro.
作者信息
Ye Liping, Jiang Yanlong, Yang Guilian, Yang Wentao, Hu Jingtao, Cui Yulin, Shi Chunwei, Liu Jing, Wang Chunfeng
机构信息
College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, 130118, China.
College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, 130118, China.
出版信息
Microb Pathog. 2017 Sep;110:325-334. doi: 10.1016/j.micpath.2017.07.015. Epub 2017 Jul 12.
Rotavirus (RV) infection causes acute, watery dehydrating diarrhea and even death in infants and other young animals, resulting in a severe economic burden; however, little is known about the innate immune mechanisms associated with RV infection. Dendritic cells (DCs), which are professional antigen-presenting cells (APCs), serve as a bridge connecting the innate and adaptive immune system. In this study, the interaction between murine bone marrow-derived DCs (BMDCs) and porcine rotavirus (PRV) was investigated in vitro. Upon stimulation, the expression levels of MHC-II, CD40, CD80, CD86 and CD83 in BMDCs increased in a time-dependent manner, indicating activation and maturation by PRV. In addition, up-regulated Toll-like receptor 2 (TLR2), TLR3 and NF-κB increased the production of interleukin-12 and interferon-γ. The PRV-stimulated BMDCs also showed increased stimulatory capacity in mixed lymphocyte reactions and promoted the Th1 subtype response.
轮状病毒（RV）感染可导致婴儿和其他幼龄动物出现急性水样脱水性腹泻甚至死亡，造成严重的经济负担；然而，关于RV感染相关的固有免疫机制，人们了解甚少。树突状细胞（DCs）作为专职抗原呈递细胞（APCs），是连接固有免疫系统和适应性免疫系统的桥梁。在本研究中，对小鼠骨髓来源的DCs（BMDCs）与猪轮状病毒（PRV）之间的相互作用进行了体外研究。受到刺激后，BMDCs中MHC-II、CD40、CD80、CD86和CD83的表达水平呈时间依赖性增加，表明PRV可使其激活并成熟。此外，Toll样受体2（TLR2）、TLR3和NF-κB的上调增加了白细胞介素-12和干扰素-γ的产生。PRV刺激的BMDCs在混合淋巴细胞反应中也表现出增强的刺激能力，并促进了Th1亚型反应。
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