Ultrasound Imaging of DNA-Damage Effects in Live Cultured Cells and in Brain Tissue.

作者信息

Tadayyon Hadi, Gangeh Mehrdad J, Vlad Roxana, Kolios Michael C, Czarnota Gregory J

机构信息

Physical Sciences, Sunnybrook Research Institute, Sunnybrook Health Sciences Centre, 2075 Bayview Avenue T2, Toronto, ON, Canada, M4N 3M5.

Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada, M4N 3M5.

出版信息

Methods Mol Biol. 2017;1644:23-40. doi: 10.1007/978-1-4939-7187-9_3.

DOI:10.1007/978-1-4939-7187-9_3

PMID:28710751

Abstract

High-frequency ultrasound (>20 MHz) spectroscopy can be used to detect noninvasively DNA damage in cell samples in vitro, and in live tissue both ex vivo and in vivo. This chapter focuses on the former two aspects. Experimental evidence suggests that morphological changes that occur in cells undergoing apoptosis result in changes in frequency-dependent ultrasound backscatter. With advances in research, ultrasound spectroscopy is advancing the boundaries of fast, label-free, noninvasive DNA damage detection technology with potential use in personalized medicine and early therapy response monitoring. Depending on the desired resolution, parametric ultrasound images can be computed and displayed within minutes to hours after ultrasound examination for cell death.

摘要