葡萄卷叶相关病毒3对本氏烟草的侵染与定殖

Infection and Colonization of Nicotiana benthamiana by Grapevine leafroll-associated virus 3.

作者信息

Prator Cecilia A, Kashiwagi Chloe M, Vončina Darko, Almeida Rodrigo P P

机构信息

Department of Environmental Science, Policy, and Management, University of California, Berkeley, CA 94720, USA.

Department of Plant Pathology, University of Zagreb Faculty of Agriculture, Zagreb, Croatia.

出版信息

Virology. 2017 Oct;510:60-66. doi: 10.1016/j.virol.2017.07.003. Epub 2017 Jul 12.

DOI:10.1016/j.virol.2017.07.003

PMID:28710957

Abstract

Grapevine leafroll disease is an increasing problem in all grape-growing regions of the world. The most widespread agent of the disease, Grapevine leafroll-associated virus 3 (GLRaV-3), has never been shown to infect species outside of the genus Vitis. Virus transmission to several plant species used as model systems was tested using the vine mealybug, Planococcus ficus. We show that GLRaV-3 is able to infect Nicotiana benthamiana. Working with GLRaV-3 infected N. benthamiana revealed distinct advantages in comparison with its natural host Vitis vinifera, yielding both higher viral protein and virion concentrations in western blot and transmission electron microscopy observations, respectively. Immunogold labelling of thin sections through N. benthamiana petioles revealed filamentous particles in the phloem cells of GLRaV-3 positive plants. Comparison of assembled whole genomes from GLRaV-3 infected V. vinifera vs. N. benthamiana revealed substitutions in the 5' UTR. These results open new avenues and opportunities for GLRaV-3 research.

摘要

葡萄卷叶病在世界上所有葡萄种植地区都是一个日益严重的问题。该病害最广泛传播的病原，葡萄卷叶相关病毒3（GLRaV-3），从未被证明能感染葡萄属以外的物种。利用葡萄粉蚧（Planococcus ficus）对几种用作模式系统的植物进行了病毒传播测试。我们发现GLRaV-3能够感染本氏烟草。与天然寄主欧洲葡萄（Vitis vinifera）相比，利用感染GLRaV-3的本氏烟草开展研究具有明显优势，在蛋白质免疫印迹和透射电子显微镜观察中，分别产生了更高浓度的病毒蛋白和病毒粒子。对本氏烟草叶柄超薄切片进行免疫金标记，在GLRaV-3阳性植株的韧皮部细胞中发现了丝状颗粒。对感染GLRaV-3的欧洲葡萄和本氏烟草的完整基因组组装结果进行比较，发现在5'UTR区域存在替换。这些结果为GLRaV-3的研究开辟了新途径和新机遇。