Zhu Jingling, Meng Pingping, Wang Qian, Wang Haiyan, Zhang Jinli, Li Yuanyuan, Li Dongzheng, Tan Xiaohua, Yang Lei, Huang Jin
The Key Laboratory of Xinjiang Endemic and Ethnic Diseases and Department of Biochemistry, Shihezi University School of Medicine, Shihezi, Xinjiang 832002, P.R. China.
Occupational and Environmental Health, Department of Preventive Medicine, School of Medicine, Hangzhou Normal University, Hangzhou, Zhejiang 310036, P.R. China.
Mol Med Rep. 2017 Sep;16(3):3201-3207. doi: 10.3892/mmr.2017.6987. Epub 2017 Jul 14.
While the neurotrophic factor neuritin is known to be involved in neurodevelopment, the effects of this compound on cell differentiation remain unclear. The present study demonstrated that neuritin treatment induced the differentiation of rat bone marrow‑derived mesenchymal stem cells (rBM‑MSCs) into neuron‑like (NL) cells. For these analyses, rBM‑MSCs were incubated with 0.5 µg/ml neuritin for 24 h. Following induction, 27% of the rBM‑MSCs exhibited typical NL cell morphologies. Subsequently, NL cells were characterized by examining the expression of neuronal markers and by analysis of cell functions. The findings demonstrated that the NL cells produced by neuritin treatment expressed the neuronal markers neuron‑specific enolase and microtubule associate protein 2, and secreted the neurotransmitter 5‑hydroxytryptamine. Furthermore, the NL cells exhibited certain partial neural‑electrophysiological functions. In conclusion, neuritin treatment may be an effective method for inducing the differentiation of BM‑MSCs towards NL cells. This may provide an alternative, potentially complementary tool for disease modeling and the development of cell‑based therapies.
虽然已知神经营养因子神经素参与神经发育，但该化合物对细胞分化的影响仍不清楚。本研究表明，神经素处理可诱导大鼠骨髓间充质干细胞（rBM-MSCs）分化为神经元样（NL）细胞。为了进行这些分析，将rBM-MSCs与0.5μg/ml神经素孵育24小时。诱导后，27%的rBM-MSCs表现出典型的NL细胞形态。随后，通过检测神经元标志物的表达和分析细胞功能对NL细胞进行表征。结果表明，经神经素处理产生的NL细胞表达神经元标志物神经元特异性烯醇化酶和微管相关蛋白2，并分泌神经递质5-羟色胺。此外，NL细胞表现出某些部分神经电生理功能。总之，神经素处理可能是诱导BM-MSCs向NL细胞分化的有效方法。这可能为疾病建模和基于细胞的治疗方法的开发提供一种替代的、潜在的补充工具。
