Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.
Shanghai Pinghe Bilingual School, Shanghai 201206, China.
Environ Pollut. 2017 Nov;230:648-654. doi: 10.1016/j.envpol.2017.07.025. Epub 2017 Jul 14.
The rapid human activities and urbanization exacerbate the human health risks induced by antibiotic resistance genes (ARGs). In this study, the profiling of ARGs was investigated using high-throughput qPCR from water samples of 13 catchment areas in East Tiaoxi River, China. High prevalence of ARGs indicated significant antibiotic resistance pollution in the research area (absolute abundance: 6.1 × 10-2.1 × 10 copies/L; relative abundance: 0.033-0.158 copies/cell). Conventional water qualities (COD, TN, TP, NH-N), bacterial communities and mobile gene elements (MGEs) were detected and analyzed as factors of ARGs shift. Nutrient and MGEs showed positive correlation with most ARGs (P < 0.05) and bacteria community was identified as the key contributing factor driving ARGs alteration. With the land-use study and field investigation, country area, especially arable, was expected as a high spot for ARGs shift and pathogen breeding. Comparing to environmental background, promotion of ARGs and marked shift of bacterial community were observed in country and urban city areas, indicating that human activities may lead to the spread of ARGs. Analysis of factors affecting ARGs in this study may shed new light on the mechanism of the maintenance and propagation of ARGs in urban rivers.
人类活动和城市化的迅速发展加剧了抗生素抗性基因（ARGs）对人类健康的威胁。本研究采用高通量 qPCR 技术，对中国东苕溪 13 个汇水区的水样中的 ARGs 进行了分析。研究结果表明，研究区域存在着严重的抗生素抗性污染（绝对丰度：6.1×10-2.1×10 拷贝/L；相对丰度：0.033-0.158 拷贝/细胞），存在大量的 ARGs（高丰度：6.1×10-2.1×10 拷贝/L；相对丰度：0.033-0.158 拷贝/细胞）。检测并分析了常规水质（COD、TN、TP、NH-N）、细菌群落和移动基因元件（MGEs）等因素对 ARGs 分布的影响。研究结果表明，营养物质和 MGEs 与大多数 ARGs 呈正相关（P<0.05），而细菌群落则被确定为驱动 ARGs 变化的关键因素。通过土地利用研究和实地调查，发现农村地区，特别是耕地，可能是 ARGs 转移和病原体滋生的高风险区域。与环境背景相比，在农村和城市地区观察到了 ARGs 的促进和细菌群落的明显变化，这表明人类活动可能导致 ARGs 的传播。本研究中对影响 ARGs 的因素的分析可能为城市河流中 ARGs 的维持和传播机制提供新的见解。
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