Hajjarian Zeinab, Tshikudi Diane M, Nadkarni Seemantini K
Wellman Center for Photomedicine, Massachusetts General Hospital and Harvard Medical School, 40 Blossom Street, Boston, MA 02114, USA.
Biomed Opt Express. 2017 Jun 30;8(7):3502-3515. doi: 10.1364/BOE.8.003502. eCollection 2017 Jul 1.
Platelets are key to maintaining hemostasis and impaired platelet aggregation could lead to hemorrhage or thrombosis. We report a new approach that exploits laser speckle intensity fluctuations, emanated from a drop of platelet-rich-plasma (PRP), to profile aggregation. Speckle fluctuation rate is quantified by the speckle intensity autocorrelation, , from which the aggregate size is deduced. We first apply this approach to evaluate polystyrene bead aggregation, triggered by salt. Next, we assess dose-dependent platelet aggregation and inhibition in human PRP spiked with adenosine diphosphate and clopidogrel. Additional spatio-temporal speckle analyses yield 2-dimensional maps of particle displacements to visualize platelet aggregate foci within minutes and quantify aggregation dynamics. These findings demonstrate the unique opportunity for assessing platelet health within minutes for diagnosing bleeding disorders and monitoring anti-platelet therapies.
血小板是维持止血的关键，血小板聚集受损可能导致出血或血栓形成。我们报告了一种新方法，该方法利用富含血小板血浆（PRP）液滴发出的激光散斑强度波动来分析聚集情况。散斑波动率通过散斑强度自相关进行量化，由此推导出聚集体大小。我们首先应用该方法评估由盐引发的聚苯乙烯珠聚集。接下来，我们评估在添加了二磷酸腺苷和氯吡格雷的人PRP中剂量依赖性的血小板聚集和抑制情况。额外的时空散斑分析产生颗粒位移的二维图，以便在数分钟内可视化血小板聚集体焦点并量化聚集动力学。这些发现证明了在数分钟内评估血小板健康状况以诊断出血性疾病和监测抗血小板治疗的独特机会。
