Peng Tao, Zhou Lixiang, Qi Hui, Wang Guangming, Luan Yongxin, Zuo Ling
1 Department of Neurosurgery, The First Hospital of Jilin University, Changchun, P.R. China.
2 Department of Ophthalmology, The Second Hospital of Jilin University, Changchun, P.R. China.
Tumour Biol. 2017 Jul;39(7):1010428317719273. doi: 10.1177/1010428317719273.
A growing body of evidence suggests that microRNA-592 is involved in tumor initiation and development in several types of human cancers. However, the biological functions and molecular mechanism of microRNA-592 in glioma remain unclear. In this study, we explored the potential role of microRNA-592 in glioma as well as the possible molecular mechanisms. Our results proved that microRNA-592 expression was significantly downregulated in glioma tissues and cell lines (p < 0.01). Functional assays revealed that overexpression of microRNA-592 dramatically reduced the cell proliferation, migration, and invasion and induced cell arrest at G1/G0 phase in vitro. Mechanistic investigations defined insulin-like growth factor binding protein 2 as a direct and functional downstream target of microRNA-592, which was involved in the microRNA-592-mediated tumor-suppressive effects in glioma cells. Moreover, the in vivo study showed that microRNA-592 overexpression produced the smaller tumor volume and weight in nude mice. In summary, these results elucidated the function of microRNA-592 in glioma progression and suggested a promising application of it in glioma treatment.
越来越多的证据表明，微小RNA-592参与了多种人类癌症的肿瘤发生和发展。然而，微小RNA-592在胶质瘤中的生物学功能和分子机制仍不清楚。在本研究中，我们探讨了微小RNA-592在胶质瘤中的潜在作用及其可能的分子机制。我们的结果证明，微小RNA-592在胶质瘤组织和细胞系中的表达显著下调（p < 0.01）。功能分析表明，微小RNA-592的过表达显著降低了体外细胞的增殖、迁移和侵袭，并诱导细胞停滞在G1/G0期。机制研究确定胰岛素样生长因子结合蛋白2是微小RNA-592直接的功能性下游靶点，其参与了微小RNA-592介导的胶质瘤细胞肿瘤抑制作用。此外，体内研究表明，微小RNA-592过表达使裸鼠的肿瘤体积和重量减小。总之，这些结果阐明了微小RNA-592在胶质瘤进展中的功能，并提示其在胶质瘤治疗中具有广阔的应用前景。
