Duarte Glennyha F, Macedo Dayane B, Luz Christian
DMIPP, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, GO, 74690-900, Brazil.
DMIPP, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, GO, 74690-900, Brazil.
Acta Trop. 2017 Oct;174:130-131. doi: 10.1016/j.actatropica.2017.07.009. Epub 2017 Jul 15.
Laboratory-reared hematophagous triatomines that are the main vectors of Chagas disease are generally fed on small vertebrates or on blood-filled membrane devices. Such devices allow a large-scale rearing of these vectors without sacrificing host animals but are almost always expensive, fragile, not easily purchased, and usually difficult to use because they need to be sterilized before and carefully cleaned after each feeding. We present here a simple device to feed triatomines that is composed of a circular series of alternating short pieces of stainless steel and silicon tubing connected to a pump that circulates water heated at 50° C in a water bath. An unlubricated condom filled with 50ml of blood, is fixed around each steel tube section and placed onto the screened tops of containers with triatomines. Nymphs and adults access the blood source by climbing a vertical piece of folded filter paper. After feeding, each condom can be easily and safely removed and discarded. This device consists of simple, cheap, safe and robust components that can be easily purchased, assembled and used. Contamination during or after feeding is practically excluded. The technique is successfully used in our Laboratory for almost two years.
实验室饲养的吸血锥蝽是恰加斯病的主要传播媒介，通常以小型脊椎动物或充满血液的膜装置为食。这类装置可在不牺牲宿主动物的情况下大规模饲养这些传播媒介，但几乎总是价格昂贵、易碎、不易购买，而且通常使用困难，因为每次喂食前都需要进行消毒，喂食后还需仔细清洁。我们在此介绍一种用于喂养锥蝽的简单装置，它由一系列交替排列的圆形短不锈钢管和硅管组成，连接到一个泵上，该泵使在50°C水浴中加热的水进行循环。一个装有50毫升血液的未润滑避孕套固定在每个钢管段周围，并放置在装有锥蝽的容器的筛网上方。若虫和成虫通过攀爬一片垂直折叠的滤纸获取血液来源。喂食后，每个避孕套都可以轻松、安全地取出并丢弃。该装置由简单、便宜、安全且坚固的部件组成，这些部件易于购买、组装和使用。喂食期间或之后的污染几乎可以排除。这项技术在我们实验室已成功使用了近两年。
