内源性转谷氨酰胺酶对乙酰胆碱受体的钙离子依赖性失活作用。
Ca2+-dependent inactivation of acetylcholine receptors by an endogenous transglutaminase.
作者信息
Hucho F, Bandini G
出版信息
FEBS Lett. 1986 May 12;200(2):279-82. doi: 10.1016/0014-5793(86)81152-8.
The nicotinic acetylcholine receptor (nAChR) from Torpedo californica and T. marmorata electric tissue polymerises irreversibly when DTE and Ca2+ are added to receptor-rich membranes. The polymerisation is time-dependent and complete within 3 h at 30 degrees C. It can be completely prevented by EGTA or the transglutaminase inhibitor cystamine. Transglutaminase activity can also be monitored with the exogenous substrates [3H]putrescine and dimethylcasein. This assay can also be inhibited by EGTA or cystamine.
当向富含受体的膜中添加二硫苏糖醇（DTE）和钙离子（Ca2+）时，来自加州电鳐和斑纹电鳐电组织的烟碱型乙酰胆碱受体（nAChR）会不可逆地聚合。这种聚合反应具有时间依赖性，在30摄氏度下3小时内完成。乙二胺四乙酸（EGTA）或转谷氨酰胺酶抑制剂胱胺可以完全阻止这种聚合反应。转谷氨酰胺酶活性也可以用外源性底物[3H]腐胺和二甲基酪蛋白进行监测。该检测方法也可被EGTA或胱胺抑制。
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