Sato H, Sato Y
Dev Biol Stand. 1985;61:461-7.
Pertussis toxin (PT) and filamentous hemagglutinin (FHA) which are the main components of pertussis vaccine in Japan, were investigated concerning their role as protective antigens. Four different experiments were carried out to investigate the ability of the antigens or antibodies to protect mice from pertussis infection: 1) Mice immunized with formalinized PT (PTd) and/or FHA (f-FHA) were tested by the intracerebral challenge system; 2) suckling mice immunized through the placenta or the milk of a mother immunized with PTd or f-FHA were tested in the aerosol challenge system; 3) suckling mice immunized by intraperitoneal injection of anti-PT and/or anti-FHA mouse antibody were tested by the aerosol challenge system; and 4) suckling mice immunized by intraperitoneal injection of monoclonal antibody against the largest subunit protein (S1) of PT were tested in the aerosol challenge system. It was concluded in all experiments performed that PTd or anti-PT antibody was a more potent protective antigen or antibody than f-FHA or its antibody, but the coexistence of both antigens or antibodies was much more potent. The synergistic effect caused by the different roles of the two antigens or antibodies was presented and discussed.
百日咳毒素（PT）和丝状血凝素（FHA）是日本百日咳疫苗的主要成分，对它们作为保护性抗原的作用进行了研究。进行了四项不同的实验来研究抗原或抗体保护小鼠免受百日咳感染的能力：1）通过脑内攻击系统对用甲醛处理的PT（PTd）和/或FHA（f-FHA）免疫的小鼠进行测试；2）通过胎盘或用PTd或f-FHA免疫的母亲的乳汁免疫的乳鼠在气溶胶攻击系统中进行测试；3）通过腹腔注射抗PT和/或抗FHA小鼠抗体免疫的乳鼠在气溶胶攻击系统中进行测试；4）通过腹腔注射抗百日咳毒素最大亚基蛋白（S1）的单克隆抗体免疫的乳鼠在气溶胶攻击系统中进行测试。在所有进行的实验中得出的结论是，PTd或抗PT抗体比f-FHA或其抗体是更有效的保护性抗原或抗体，但两种抗原或抗体的共存则更有效。提出并讨论了两种抗原或抗体不同作用所产生的协同效应。
