Ghosh B B, Banerjee A K
Folia Microbiol (Praha). 1986;31(2):106-12. doi: 10.1007/BF02926827.
A hydrocarbon-utilizing Serratia marcescens var. kiliensis grew and accumulated methionine and glutamic acid in a synthetic medium with hydrocarbon as sole carbon source. n-Hexadecane and ammonium phosphate were found as the most suitable carbon and nitrogen sources, respectively. Optimum pH for growth and methionine production was 7.2, and that for glutamic acid accumulation was 7.4. Yeast extract significantly stimulated growth and amino acid production and could be substituted by cyanocobalamine. Benzylpenicillin, Tween 80, SDS or EDTA did not increase amino acid production. Under optimal cultural conditions in the laboratory the organism produced 1.68 g of glutamic acid and 0.78 g of methionine per litre.
一种利用烃类的粘质沙雷氏菌基利恩斯变种在以烃类作为唯一碳源的合成培养基中生长，并积累蛋氨酸和谷氨酸。正十六烷和磷酸铵分别被发现是最合适的碳源和氮源。生长和蛋氨酸生产的最适pH值为7.2，谷氨酸积累的最适pH值为7.4。酵母提取物显著促进生长和氨基酸生产，并且可以用氰钴胺替代。苄青霉素、吐温80、十二烷基硫酸钠或乙二胺四乙酸不会增加氨基酸产量。在实验室的最佳培养条件下，该生物体每升产生1.68克谷氨酸和0.78克蛋氨酸。
