Werner H, Okon E, Fridkin M, Koch Y
J Clin Endocrinol Metab. 1986 Jul;63(1):47-50. doi: 10.1210/jcem-63-1-47.
The localization of the recently identified GH-releasing hormone (GHRH) in the human hypothalamus and pituitary stalk was determined by microdissection techniques and a specific RIA for GHRH. The highest concentrations of GHRH immunoreactivity (IR-GHRH) in the hypothalamus were found in the area of the infundibular nucleus (83 +/- 4 ng/mg protein; average +/- range). Lower quantities were found in other hypothalamic regions. Very high concentrations of IR-GHRH were present in the upper portion of the pituitary stalk (1454 +/- 48 ng/mg protein), and they decreased gradually toward the distal end of the stalk (21 +/- 3 ng/mg). This concentration gradient suggests that the peptide reaches the anterior pituitary mainly by way of the long portal vessels. Somatostatin, the second neuropeptide involved in the regulation of GH secretion from the anterior pituitary, had a pattern of distribution along the pituitary stalk very similar to that of IR-GHRH.
通过显微解剖技术和针对生长激素释放激素（GHRH）的特异性放射免疫分析（RIA），确定了最近发现的GHRH在人下丘脑和垂体柄中的定位。下丘脑内GHRH免疫反应性（IR - GHRH）浓度最高的区域位于漏斗核区域（83±4 ng/mg蛋白质；平均值±范围）。在下丘脑其他区域发现的量较低。垂体柄上部存在非常高浓度的IR - GHRH（1454±48 ng/mg蛋白质），并且它们朝着柄的远端逐渐降低（21±3 ng/mg）。这种浓度梯度表明该肽主要通过长门静脉到达垂体前叶。生长抑素是参与调节垂体前叶生长激素分泌的第二种神经肽，其沿垂体柄的分布模式与IR - GHRH非常相似。
