A Ligand-Based NMR Screening Approach for the Identification and Characterization of Inhibitors and Promoters of Amyloid Peptide Aggregation.

Over the years a significant amount of effort has been put into the development of rapid and reliable methods to monitor the aggregation dynamics of the β amyloid peptide in real time. We present an alternative approach based on a suitable reporter or spy molecule and three different NMR experiments: WaterLOGSY, H selective T filter, and F T filter, for monitoring the initial self-aggregation process kinetics of the β amyloid peptide and identifying molecules that retard or accelerate the self-aggregation process. Although the proposed method is not a high-throughput assay, it avoids problems associated with interference events that are sometimes observed in fluorescence-based assays.