Orthobiologic Laboratory, MedStar Union Memorial Hospital, 201 E. University Parkway, Bauernschmidt Building 763, Baltimore, MD, 21228, USA.
School of Medicine, Saint Louis University, St. Louis, MO, USA.
Calcif Tissue Int. 2017 Nov;101(5):549-552. doi: 10.1007/s00223-017-0306-z. Epub 2017 Jul 19.
This study described and histologically characterized a cyst-like lesion (CLL) at the chondro-osseous junction. Rat knees (n = 12), with or without excessive running-induced osteoarthritis (OA), were used for counting the incidence, morphological measurements, immunohistochemistry of the CLL. A typical CLL, appearing as a void space in the matrix, was located on the tidemark at the chondro-osseous junction. The content of the CLL included types II and VI collagen, proteoglycans but not intact chondrocytes. At least one CLL was found in 5/6 osteoarthritic knees and only 2/6 in the non-osteoarthritic knees. The margin of the CLL was depleted of proteoglycans. The chondrocytes around the lesion were deformed and occasionally apoptotic. Matrix metalloproteinase 13 and vascular endothelial growth factor receptor were not detected in and around the CLL. CLLs disrupt the integrity of cartilage at a mechanically critical location-the chondro-osseous junction. The significance of the CLL in cartilage biology and its potential role in OA pathogenesis warrant further investigation.
本研究描述并组织学表征了软骨-骨交界处的囊样病变（CLL）。使用大鼠膝关节（n=12），有无过度跑步引起的骨关节炎（OA），用于计算 CLL 的发生率、形态学测量、免疫组织化学。CLL 出现在软骨-骨交界处的钙化带处，表现为基质中的空洞。CLL 的内容物包括 II 型和 VI 型胶原、蛋白聚糖，但不包括完整的软骨细胞。在 5/6 例骨关节炎膝关节中发现至少一个 CLL，而在非骨关节炎膝关节中仅发现 2/6 例。CLL 的边缘缺乏蛋白聚糖。病变周围的软骨细胞变形，偶尔出现凋亡。CLL 内和周围未检测到基质金属蛋白酶 13 和血管内皮生长因子受体。CLL 在机械上关键位置-软骨-骨交界处破坏了软骨的完整性。CLL 在软骨生物学中的意义及其在 OA 发病机制中的潜在作用值得进一步研究。
