维生素D缺乏和补充维生素D的大鼠肝脏微粒体胆钙化醇25-羟化酶的动力学

Kinetics of liver microsomal cholecalciferol 25-hydroxylase in vitamin D-depleted and -repleated rats.

作者信息

Delvin E E, Arabian A, Glorieux F H

出版信息

Biochem J. 1978 Jun 15;172(3):417-22. doi: 10.1042/bj1720417.

DOI:10.1042/bj1720417

PMID:28728

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1185715/

Abstract

Kinetics of vitamin D-depleted and -repleted rat liver microsomal cholecalciferol 25-hydroxylase were studied. Anaerobiosis, CO, omission of a NADPH-generating system and addition of detergents all decreased the activities, showing that the hydroxylase behaves like a cytochrome P-450-dependent enzyme. An apparent Km of 0.18 micrometer and Vmax. of 32pmol/min per g of tissue were found for vitamin D-deficient animals. Although both apparent Km and Vmax. were significantly altered in vitamin D-repleted animals no inhibition of the enzyme was elicited. These latter results show that at normal vitamin D intake, rat liver cholecalciferol 25-hydroxylase is not feedback-inhibited.

摘要

研究了维生素D缺乏和补充的大鼠肝脏微粒体胆钙化醇25 -羟化酶的动力学。厌氧、一氧化碳、缺乏NADPH生成系统以及添加去污剂均降低了酶活性，表明该羟化酶表现得像一种细胞色素P - 450依赖性酶。维生素D缺乏的动物中，维生素D的表观Km为0.18微米，Vmax为每克组织32皮摩尔/分钟。尽管在维生素D补充的动物中表观Km和Vmax均有显著改变，但未引起该酶的抑制。后一结果表明，在正常维生素D摄入量时，大鼠肝脏胆钙化醇25 -羟化酶不受反馈抑制。

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