产气荚膜梭菌溶血素O对人红细胞膜磷脂酸代谢的激活作用。

Activation of phosphatidic acid metabolism of human erythrocyte membranes by perfringolysin O.

作者信息

Saito M, Ando S, Mitsui K, Homma Y, Takenawa T

出版信息

Biochem Biophys Res Commun. 1986 May 29;137(1):23-8. doi: 10.1016/0006-291x(86)91170-8.

Abstract

The effect of perfringolysin O on the lipid metabolism of human erythrocyte membranes was investigated. Erythrocytes were prelabeled with [3H]arachidonic acid and [32P]inorganic phosphate. In the presence of calcium ion(5.5 mM), the effect of perfringolysin O on lipid metabolism was very similar to that of an calcium-ionophore A23187. In the absence of calcium ion, the accumulation of phosphatidic acid and its following decreasing trend were observed during the reaction with the toxin. Such changes were not caused by filipin. These results suggest that perfringolysin O causes the activation of a diglyceride-phosphatidic acid cycle, which might be involved in the calcium transport.

摘要

研究了产气荚膜梭菌溶素O对人红细胞膜脂质代谢的影响。红细胞用[3H]花生四烯酸和[32P]无机磷酸盐进行预标记。在钙离子（5.5 mM）存在的情况下，产气荚膜梭菌溶素O对脂质代谢的影响与钙离子载体A23187非常相似。在没有钙离子的情况下，在与毒素反应过程中观察到磷脂酸的积累及其随后的下降趋势。这些变化不是由制霉菌素引起的。这些结果表明，产气荚膜梭菌溶素O导致甘油二酯-磷脂酸循环的激活，这可能与钙转运有关。