Ejiri K, Taniguchi H, Ishihara K, Hirose Y, Terashi K, Murakami K, Imamura Y, Baba S
Diabetes Res Clin Pract. 1985 Aug;1(2):73-9. doi: 10.1016/s0168-8227(85)80031-0.
Tris(hydroxymethyl)aminomethane (Tris) has been shown to inhibit selectively the Golgi apparatus and Golgi-endoplasmic reticulum-lysosomal system (GERL system) of several kinds of cells including pancreatic B cells. This study was designed to assess the effect of Tris on insulin, glucagon and somatostatin release and insulin synthesis in pancreatic B cells by using isolated rat pancreatic islets. Tris suppressed glucose-induced insulin release, whereas it did not affect the glucagon and somatostatin release. Furthermore, the incorporation of [3H]leucine into the insulin fraction was suppressed by 10 mM Tris, but the sum of the radioactivity of both proinsulin and insulin fraction were not influenced. The present study suggests that the Golgi apparatus and GERL system may play a role in insulin secretion and biosynthesis in pancreatic B cells.
已证实三（羟甲基）氨基甲烷（Tris）可选择性抑制包括胰腺β细胞在内的多种细胞的高尔基体以及高尔基体 - 内质网 - 溶酶体系统（GERL系统）。本研究旨在通过使用分离的大鼠胰岛来评估Tris对胰腺β细胞中胰岛素、胰高血糖素和生长抑素释放以及胰岛素合成的影响。Tris抑制了葡萄糖诱导的胰岛素释放，而对胰高血糖素和生长抑素的释放没有影响。此外，10 mM Tris抑制了[3H]亮氨酸掺入胰岛素组分，但胰岛素原和胰岛素组分的放射性总和未受影响。本研究表明，高尔基体和GERL系统可能在胰腺β细胞的胰岛素分泌和生物合成中发挥作用。
