Fujita Y, Amemiya H, Abe Y, Takada I, Ishii K, Yajima Y, Yamamoto N, Miyazawa H, Okabe H
Diabetes Res Clin Pract. 1985;1(6):335-42. doi: 10.1016/s0168-8227(86)80046-8.
Insulin, glucagon and somatostatin (SLI) in the pancreas and the gastrointestinal tissue of rats were measured following a high (300 mg/kg) or low (150 mg/kg) dose of cysteamine, given intermittently for 14 days. In addition, the effect of prolonged cysteamine-induced depletion of pancreatic SLI upon the cell distribution within the Langerhans islets was compared with that of chronic insulin-deficient rats produced by streptozotocin. The high or low dose of cysteamine reduced pancreatic SLI to 8.3% and gastrointestinal SLI to 5.6% of control levels without duodenal ulcer. The high dose of cysteamine also reduced pancreatic insulin to 37% of controls without hyperglycemia. No change in the glucagon concentration was observed. In SLI-deficient rats, distribution of A and B cells was similar to that of controls, even though D cells were rarely seen. In insulin-deficient rats, however, the number of A and D cells per islet area increased with a concomitant decrease in B cells. Intermittent administration of a low dose of cysteamine, thus, appears to be useful to produce a chronic SLI-deficient rat. However, a high dose of cysteamine is not a specific depletor of pancreatic SLI. Although insulin may be important to maintain normal cell distribution within the islets, pancreatic SLI may not have such a role.
在大鼠间歇性给予高剂量（300毫克/千克）或低剂量（150毫克/千克）半胱胺14天后，测定其胰腺和胃肠道组织中的胰岛素、胰高血糖素和生长抑素（SLI）。此外，将半胱胺诱导的胰腺SLI长期耗竭对胰岛内细胞分布的影响与链脲佐菌素诱导的慢性胰岛素缺乏大鼠的影响进行了比较。高剂量或低剂量的半胱胺可将胰腺SLI降低至对照水平的8.3%，胃肠道SLI降低至对照水平的5.6%，且无十二指肠溃疡。高剂量的半胱胺还可将胰腺胰岛素降低至对照水平的37%，但无高血糖。未观察到胰高血糖素浓度的变化。在SLI缺乏的大鼠中，A细胞和B细胞的分布与对照相似，尽管很少见到D细胞。然而，在胰岛素缺乏的大鼠中，每个胰岛区域的A细胞和D细胞数量增加，同时B细胞数量减少。因此，间歇性给予低剂量的半胱胺似乎有助于制备慢性SLI缺乏的大鼠。然而，高剂量的半胱胺不是胰腺SLI的特异性耗竭剂。虽然胰岛素可能对维持胰岛内正常细胞分布很重要，但胰腺SLI可能没有这样的作用。
