化学修饰RNA纳米颗粒的共转录生产
Cotranscriptional Production of Chemically Modified RNA Nanoparticles.
作者信息
Kireeva Maria L, Afonin Kirill A, Shapiro Bruce A, Kashlev Mikhail
机构信息
RNA Biology Laboratory, National Cancer Institute, National Institutes of Health, Frederick, MD, 21702, USA.
Nanoscale Science Program, Department of Chemistry, University of North Carolina at Charlotte, 9201 University City Boulevard, Charlotte, NC, USA.
出版信息
Methods Mol Biol. 2017;1632:91-105. doi: 10.1007/978-1-4939-7138-1_6.
RNA nanoparticles consisting of multiple RNA strands of different sequences forming various three-dimensional structures emerge as promising carriers of siRNAs, RNA aptamers, and ribozymes. In vitro transcription of a mixture of dsDNA templates encoding all the subunits of the RNA nanoparticle may result in cotranscriptional self-assembly of the nanoparticle. Based on our experience with production of RNA nanorings, RNA nanocubes, and RNA three-way junctions, we propose a strategy for optimization of the cotranscriptional production of chemically modified ribonuclease-resistant RNA nanoparticles.
由不同序列的多条RNA链组成各种三维结构的RNA纳米颗粒，正成为小干扰RNA（siRNA）、RNA适配体和核酶的有前景的载体。对编码RNA纳米颗粒所有亚基的双链DNA模板混合物进行体外转录，可能会导致纳米颗粒的共转录自组装。基于我们在生产RNA纳米环、RNA纳米立方体和RNA三向接头方面的经验，我们提出了一种优化化学修饰的抗核糖核酸酶RNA纳米颗粒共转录生产的策略。
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