Matic Stevan D, Rakocevic Milena S, Jocic Tomislav T, Todorovic Milos S, Vuckovic Ljiljana M, Jancic Snezana A, Knezevic Milan G
Department of Pathology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia.
J BUON. 2017 May-Jun;22(3):757-765.
To investigate the microvessel density (MVD) and proliferation in prostate cancer (PC) core biopsies.
Core biopsy samples of PC tissue from 45 patients were routinely processed and embedded in paraffin. The samples of PC formed the investigated group (n=25), while samples of benign prostatic hyperplasia (BPH) served as controls (n=20). From paraffin blocks, 3-5 μm-thick sections were made and routine hematoxylin-eosin method and immunohistochemical ABC method with Ki67 and CD34 antibodies were applied. Immunohistochemical expression of Ki67 and CD34 was stereometrically quantified.
The median number of Ki67 and CD34 positive cells per mm2 in PC were significantly higher in comparison to the median of these cells in BHP. The average age and Gleason score in patients with high proliferation index (proIDX) and MVD index (mvdIDX) was significantly greater in comparison to those with low proIDX and low mvdIDX. The absolute values of Ki67 expression were in highly positive and significant correlation with the absolute values of CD34 expression. Highly significant correlation was found between Gleason score and proIDX and mvdIDX.
This study showed that PC expressed significantly higher values of Ki67 and CD34 in comparison to BPH. The values of proIDX and mvdIDX obtained by core biopsy could clearly show the level of cancer progression expressed through highly correlated Gleason score. In this way it is possible to identify the patients at high risk for disease progression.
研究前列腺癌（PC）穿刺活检组织中的微血管密度（MVD）及增殖情况。
对45例患者的PC组织穿刺活检样本进行常规处理并石蜡包埋。PC样本组成研究组（n = 25），良性前列腺增生（BPH）样本作为对照组（n = 20）。从石蜡块上制作3 - 5μm厚的切片，采用常规苏木精 - 伊红染色法以及用Ki67和CD34抗体的免疫组织化学ABC法。对Ki67和CD34的免疫组织化学表达进行立体定量分析。
与BPH相比，PC中每平方毫米Ki67和CD34阳性细胞的中位数显著更高。高增殖指数（proIDX）和MVD指数（mvdIDX）患者的平均年龄和Gleason评分显著高于低proIDX和低mvdIDX患者。Ki67表达的绝对值与CD34表达的绝对值呈高度正相关且具有统计学意义。Gleason评分与proIDX和mvdIDX之间存在高度显著的相关性。
本研究表明，与BPH相比，PC中Ki67和CD34的表达值显著更高。通过穿刺活检获得的proIDX和mvdIDX值能够清晰显示通过高度相关的Gleason评分所表达的癌症进展水平。通过这种方式，有可能识别出疾病进展风险高的患者。
